摘要
目的探讨临床常规血小板(PLT)计数不准确的原因,并提出纠正措施。方法收集PLT计数异常标本180例,分为4组,分别为低值PLT组(PLT≤20×109/L)72例,小红细胞组54例,大PLT组33例,PLT聚集组21例,分别采用光学法(PLT-O)、阻抗法(PLT-I)、手工镜检法(PLT-M)3种方法同时计数PLT,用配对t检验进行分析。结果低值PLT组、小红细胞组、大PLT组光学法与PLT计数PLT-M法比较差异无统计学意义(P>0.05),而PLT-I法与PLT-M法PLT计数比较差异有统计学意义(P<0.05)。PLT聚集组的标本,PLT-I法和PLT-O法计数结果比较,PLT-O法结果更接近真实值。结论 PLT-I法PLT计数的影响因素很多,主要有小红细胞干扰导致计数偏高,大PLT漏检导致PLT结果偏低,PLT聚集等,当PLT计数结果异常时应根据复检规则复检,采用手工PLT-M法或者PLT-O法纠正,必要时重新采血测定。
Objective To investigate the inaccurate causes of platelet（PLT）count and to propose the correcting measures.Meth-ods 180 specimens of abnormal PLT count were collected and divided into 4 groups：low PLT count group （PLT ≤20×109/L,72 cases）,microcyte group（54 cases）,large platelets group（33 cases）and platelet aggregation group（21 cases）.The PLT count was simultaneously performed by using the three ways of the optical method （PLT-O）,impedance method （PLT-I）and manual micros-copy method （PLT-M）.The detection results were analyzed by the paired t test.Results The PLT count in the low PLT group, microcyte group and large platelets group had no statistical differences between PLT-O and PLT-M（P 〉0.05）,and had statistical differences between the PLT-I and PLT-O（P 〈0.05）.The PLT count of PLT-O in the platelet aggregation group was more close to actual value than that of PLT-I（P 〈0.05）.Conclusion The PLT count by PLT-I has many influencing factors,which are mainly the microcytes interference leading to higher PLT count,missed detection of large PLT leading to lower PLT count,PLT aggrega-tion,etc.When the PLT count appears abnormal,the re-detection should be conducted according to the re-detection regulation, which can be corrected by adopting PLT-M or PLT-O,in necessity,the blood specimen could be re-collected for detection.
出处
《国际检验医学杂志》
CAS
2014年第20期2816-2817,共2页
International Journal of Laboratory Medicine
关键词
血小板计数
光学法
影响因素
复检规则
platelet count
optical method
influencing factors
re-detection regulation
