摘要
目的:探讨低剂量紫杉醇对膀胱出口部分梗阻(BOO)大鼠膀胱形态变化的影响。方法:健康雌性Sprague-Dawley大鼠30只,随机分为空白对照组、BOO组和紫杉醇组。BOO组和紫杉醇组行尿道近端梗阻方式制备膀胱出口部分梗阻模型,空白对照组采用假手术方式处理。紫杉醇组于术后给予紫杉醇(溶于生理盐水)腹腔注射(0.3 mg/kg),每周2次。空白对照组与BOO组以等体积生理盐水同时间腹腔注射。4周后测量体重及膀胱重量,HE染色观察膀胱肌层增生情况;Masson三色染色观察并评价胶原纤维沉积;透射电镜观察逼尿肌超微结构改变。结果:与空白对照组相比,BOO组膀胱重量明显增加(0.376 g±0.052 g vs 0.112 g±0.014 g,P<0.05),膀胱逼尿肌明显增厚,胶原纤维面积占胶原与肌组织面积之和的比值下降(29.66%±2.69%vs 38.94%±3.67%,P<0.05),电镜下细胞中间连接明显减少,出现丰富的突触连接与桥粒连接,肌细胞间隙内有大量的胶原纤维。与BOO组相比,紫杉醇组膀胱重量显著降低(0.215 g±0.025 g vs 0.376 g±0.052 g,P<0.05),膀胱逼尿肌厚度减小,胶原纤维染色面积占胶原与肌组织面积之和的比值下降(19.94%±1.90%vs 29.66%±2.69%,P<0.05),细胞间连接以中间连接为主,细胞间隙内胶原纤维减少。结论:低剂量紫杉醇对BOO后膀胱形态结构的变化有一定的修复或逆转作用。
AIM： To investigate the effects of low-dose paclitaxel on the morphology of bladder after partial bladder outlet obstruction（ BOO） in rats. METHODS： Healthy female Sprague-Dawley rats（ n = 30） were randomly divided into sham operation group,BOO group and low-dose paclitaxel group. The rats in BOO group and low-dose paclitaxel group received operation to establish an obstruction model,while the rats in sham group underwent sham operation. After operation,the rats in low-dose paclitaxel group received intraperitoneal injection of paclitaxel at a dose of 0. 3 mg /kg twice a week for 4 weeks. At the same time,the animals in sham group and BOO group received the same volume of saline by intraperitoneal injection. Four weeks after operation,each rat was sedated and the bladder was weighted. Histological changes of the bladder were observed by HE staining. Collagen deposition in the bladder tissue was observed by Masson staining,and the fibrosis area was measured. The ultrastructure of the detrusor was studied by transmission electron microscopy. RESULTS： Compared with sham operation group,a significant increase in bladder weight（ 0. 376 g ± 0. 052 g vs0. 112 g ± 0. 014 g,P 0. 05）,the muscle hypertrophy,and a decrease in the percentage of collagen area [collagen /（ collagen ＋ muscle）,29. 66% ± 2. 69% vs 38. 94% ± 3. 67%,P 0. 05]was observed in BOO group. Under electron microscope,intracellular connection had more gap junction and desmosomes than intermediate junction. The cell gap widened with a large amount of collagen fiber. Compared with BOO group,low-dose paclitaxel group decreased bladder weight（ 0.215 g ±0.025 g vs 0.376 g ±0.052 g,P 〈0.05） and improved the muscle hypertrophy. The percentage of the collagen area was also decreased（ 19. 94% ± 1. 90% vs 29. 66% ± 2. 69%,P〈 0. 05）. The detrusor microstructure showed that the intermediate junction was characterized by a predominance among the intracellular connections,and the intercellular space contained less collagen fibers in low-dose paclitaxel group. CONCLUSION： Low-dose paclitaxel may ameliorate the morphological damage of the bladder and recover bladder function in the rats with BOO by slowing down the process of bladder fibrosis.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2014年第10期1896-1901,共6页
Chinese Journal of Pathophysiology
基金
徐州市科技发展基金资助项目(No.XF11C066)
关键词
膀胱出口梗阻
紫杉醇
透射电子显微镜
Bladder outlet obstruction
Paclitaxel
Transmission electron microscope
