摘要
目的:研究DNA依赖蛋白激酶(DNA-PK)在人喉鳞状细胞癌(LSCC)及正常喉黏膜组织中的表达,探讨其与LSCC临床病理特征之间的关系。方法:应用免疫组织化学方法(Envision)检测64例LSCC组织(实验组)及15例正常喉黏膜组织(对照组)中DNA-PK的表达水平,结合LSCC的临床病理特征进行分析。结果:DNA-PK在对照组和实验组中的阳性表达率分别为26.67%(4/15)、78.13%(50/64),差异有统计学意义(P<0.05)。DNA-PK表达与LSCC的组织分化程度相关(P<0.05),与临床分期、淋巴结转移无明显相关(P>0.05)。结论:DNA-PK的表达与LSCC的病变发展有关。
Objective：To study the expression of DNA-dependent protein kinase（DNA-PK）in human laryngeal squamous cell carcinoma（LSCC）and normal laryngeal mucosa（NLM）,and to analysize the relationship between the expression and the clinicopathologic parameters of LSCC.Method：Immunohistochemical technique（Envision）was used to detect the expression of DNA-PK in 64 cases of LSCC and 15 cases of NLM.To investigateAn investigation was conducted on the relationship between the expression and clinico-pathological features of LSCC.Result：DNA-PK was lowly expressed in NLM and highly expressed in LSCC,the positive rate of DNA-PK expression was 26.67%（4/15）,78.13%（50/64）,respectively,and there was significant different difference between the two groups（P〈0.05）.Its expression was correlated with the level of histodifferentiation（P〈0.05）,but not with TNM stages and neck lymph node metastasis（P〈0.05）.Conclusion：DNA-PK may be involved in disease development of LSCC.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
北大核心
2014年第21期1641-1644,共4页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
基金
贵州省科技联合基金[No:黔科合LS字(2011)016号]
关键词
喉黏膜
喉鳞状细胞癌
DNA依赖蛋白激酶
免疫组织化学
laryngeal mucosa
laryngeal squamous cell carcinoma
DNA-dependent protein kinase
immunohis-tochemlstry
