摘要
目的:研究乌鲁木齐地区耐甲氧西林金黄色葡萄球菌(MRSA)的分子分型及其耐药情况。方法:收集金黄色葡萄球菌163株,PCR方法扩增mecA及pvl基因,检测MRSA及pvl检出率,用SCCmec、spa、MLST等方法对MRSA进行分型,VITEK2进行药敏测定。结果:MRSA检出率39.3%,pvl阳性率45.3%,HA-MRSA为87.5%,CA-MRSA为10.9%,未定型MRSA1株。有SCCmec型5种、spa型12种及ST型6种,其中HA-MRSA以ST239-MRSA-Ⅲ-t030、ST239-MRSA-Ⅲ-t037和ST5-MRSA-Ⅱ-t002为主,CA-MRSA以ST59-MRSA-Ⅳ-t437为主,耐药结果显示未发现耐万古霉素、替考拉宁、利奈唑胺等MRSA。结论:乌鲁木齐地区MRSA较低,以HA-MRSA为主,MRSA的多种药物敏感性呈下降趋势,值得重视。
Objective：To investigate the molecular types and drug susceptibilities of methicillin-resistant Staphylococcus aureus prevailed in Urumqi area. Methods： A total of 163 S. aureus isolates were collected between March 2011 and March 2012 in our hospital. PCR amplification was performed to detect the MRSA and pvl genes. SCCmec typing, spa typing,and MLST were performed to detect the molecular types for all MRSA isolates. And the drug susceptibilities to 15 types of antibiotics were also analyzed. Results： Among these 163 S. aureus isolates, there were 64 MRSA isolates including 56 HA-MRSA,7 CA-MRSA,1 non typeable MRSA,and 29 PVL-positive MRSA isolates. In these MRSA isolates, 12 spa types and 6 ST were found. The predominant HA- MRSA clones were ST239- MRSA- Ⅲ - t030, ST239 - MRSA- Ⅲ -t037 and ST5- MRSA- Ⅱ - t002, and ST59 - MRSA- Ⅳ - t437 was the predominant CA- MRSA. Conclusion：Among the isolates collected in our hospital, 64 were characterized as MRSA, with a lower prevalence of 39.3%. HA-MRSA is still the most predominant MRSA in Urumqi area,and CA-MRSA was also found. The antibiotic susceptibility of MRSA is decreasing,it is the time to monitor the prevalence of drug resistance of MRSA.
出处
《西北国防医学杂志》
CAS
2014年第5期449-452,共4页
Medical Journal of National Defending Forces in Northwest China
