摘要
以丙烯腈为底物,对产腈水解酶菌株Pseudomonas putida XY4在静息细胞状态下的催化特性进行了研究,考察了p H、温度、金属离子、底物浓度对酶的影响,以及该酶的p H与温度稳定性、底物特异性等,同时对丙烯腈的生物转化过程进行考察。结果表明,腈水解酶的最佳催化条件为p H=7.2,30℃,底物浓度150 mmol/L;Ag+、Cu2+和Fe3+对酶具有强烈的抑制作用;该腈水解酶对3-氰基吡啶等芳香族杂环腈具有较高的特异性,其次是甘氨腈和丙烯腈,对酰胺类物质无特异性。该研究对利用腈水解酶生物催化丙烯腈制备丙烯酸的应用提供借鉴。
The catalytic properties of nitrilase produced by Pseudomonas putida XY4 were studied with acrylonitrile as the substrate. The effects of pH, temperature, metal ions, and the substrate concentration on the enzymatic activity were investigated. Besides, pH and temperature stability and the substrate specificity were studied. The biotransformation process of acrylonitrile was investigated simultaneously. The optimal catalytic conditions were as follows： pH = 7.2,30 ℃, and 150 mmol/L acrylonitrile. The metal ions of Ag^ ＋ , Cu^2＋ and Fe^3＋ showed strong inhibition effect on the enzymatic activity. This nitrilase showed high specificity for aromatic heterocyclic nitriles, followed by glycinonitrile and acrylonitrile. No amidase activity was detected. This study provided a reference for the application of bio-transformation of acrvlonitrile to acrylic acid.
出处
《精细化工》
EI
CAS
CSCD
北大核心
2014年第12期1466-1470,共5页
Fine Chemicals
基金
国家高技术研究发展计划(2012AA022204C)
江苏省自然科学基金(BK20140133)~~
关键词
丙烯酸
腈水解酶
催化特性
恶臭假单胞菌
生物工程
acrylic acid
nitrilase
catalytic properties
Pseudomonas putida
biological engineering
