摘要
为筛选出红花山玉兰组织培养适宜的外植体类型及其消毒方法,为红花山玉兰的无性繁殖提供技术支持,对灭菌时间、外植体类型和取材时间等进行研究。结果表明:茎段和顶芽的存活率分别为40.0%和33.33%,较叶片和叶柄的存活率高,且茎段和顶芽的存活率较叶片的存在显著差异,二者的死亡率均与叶柄和叶片的存在显著差异;茎段和顶芽为适宜的外植体。茎段在6月取材优于12月,且采用0.1%的升汞消毒10min,其存活率为40.0%;顶芽在6月和12月取材均可,消毒后去除托叶能够有效地降低顶芽的污染率,提高其存活率。最佳消毒组合:先用75%的酒精表面喷洒预处理,再用0.1%的升汞消毒10min,之后去除顶芽的托叶。该方法污染率和死亡率低,存活率最高,依次分别为6.67%、7.78%和85.55%,且芽启动率高。
In order to select appropriate disinfection methods and explants type of M.delavayiin tissue culture,and provide technical support for its asexual propagation,the time of explants disinfection,explants types and sampling time were studied.Results：The stem and terminal bud were much better than leaves and petioles for disinfection in view of its lower rate of mortality and relatively higher survival rate.The survival rate was 40.0% and 33.33% relatively for stems and terminal buds,and the difference in mortality was significant compared with the leaves and petioles.The stems sampled in June were better than in December,and the survival rate was 40% by immersing in HgCl2（0.1%）for 10 minutes.It was possible to sample in June and December for the terminal buds,and it can effectively reduce the rate of contamination and mortality,and improved survival rate after disinfection to remove stipules.The optimal disinfection schemes was that the terminal bud were sprayed with 75% alcohol,and were immersed in HgCl2（0.1%）for 10 minutes,finally removed the terminal bud stipules,the lowest rate of contamination（6.67%）and mortality（7.78%）,and the highest survival rate was 85.55%,and the percent germination was high.
出处
《贵州农业科学》
CAS
北大核心
2014年第11期42-45,共4页
Guizhou Agricultural Sciences
基金
江西省观赏植物遗传改良重点实验室开放基金项目"红花山玉兰引种栽培及快繁技术研究"(2012-KLB-4)
关键词
红花山玉兰
外植体消毒
消毒方法
存活率
Magnolia delavayi
explants disinfection
sterilization way
survival rate
