摘要
目的探讨自身抗体和免疫功能指标对诊断原发性胆汁性肝硬化(PBC)及评估病情的价值。方法检测98例PBC患者(PBC组)和80例健康者(对照组)的抗线粒体抗体M2亚型抗体(AMA-M2)、抗三联体(抗-3E/BPO)抗体、抗可溶性酸性磷酸化核蛋白SP100(抗-SP100)抗体、抗早幼粒细胞白血病(抗-PML)抗体、抗核包膜蛋白gp210(抗-gp210)抗体。对比PBC患者中AMA-M2阳性和AMA-M2阴性患者的TBIL、ALB、TP、AST、ALT、GGT、ALP、Ig A、Ig G、Ig M水平。结果 PBC组AMA-M2、抗-3E/BPO抗体、抗-SP100抗体、抗-PML抗体、抗-gp210抗体检出率分别为66.33%、80.61%、28.57%、27.55%、38.78%,均明显高于对照组的16.25%、12.50%、2.50%、1.25%、2.50%(P<0.05)。PBC患者中AMA-M2阳性78例(79.59%),AMA-M2阴性20例(20.41%);AMA-M2阳性组ALB、TP、AST、ALT、GGT、ALP水平明显高于AMA-M2阴性组(P<0.05);两组TBIL、Ig G、Ig M、Ig A水平比较,差异无统计学意义(P>0.05)。结论检测AMA-M2水平对诊断原发性胆汁性肝硬化及评估患者病情具有重要意义,在AMA-M2呈阴性时联合检测抗-gp210抗体、抗-SP100抗体和抗-PML抗体,可以协助诊断该病。
Objective To evaluate autoantibodies and immune function indicators in the diagnosis and assessment of primary biliary cirrhosis（ PBC）. Methods Ninty-eight cases of PBC（ cirrhosis group） and 80 healthy cases（ control group） were enrolled in the study. Their anti-mitochondrial antibody M2（ AMA-M2）,anti-triad（ anti-3E / BPO） antibody,anti-phospho-soluble acidic nuclear protein SP100（ anti-SP100） antibody,anti-promyelocytic leukemia（ anti-PML） antibody,and anti-nuclear envelope protein gp210（ anti-gp210） antibody were detected. The levels of TBIL,ALB,total protein（ TP）,AST,ALT,glutamyl endopeptidase（ GGT）,alkaline phosphatase（ ALP）,Ig A,Ig G and Ig M were compared between AMA-M2 positive and negative patients with PBC. Results The detection rates of AMA-M2,anti-3E / BPO antibody,anti-SP100 antibody,anti-PML antibody,anti-gp210 antibody in the cirrhosis group were 66. 33%,80. 61%,28. 57%,27. 55%,38. 78%,respectively,which were significantly higher than those（ 16. 25%,12. 50%,2. 50%,1. 25%,2. 50%） in the control group（ P〈0. 05）. Among the patients with PBC,positive AMA-M2 amounted to 78 cases（ 79. 59%）,negative AMA-M2 amounted to 20 cases（ 20. 41%）;The levels of ALB,TP,AST,ALT,GGT,ALP in the positive AMA-M2 group were significantly higher than those in the negative AMA-M2 group（ P〈0. 05）; There was no significant difference in the levels of TBIL,Ig G,Ig M,Ig A between two groups（ P〉0. 05）. Conclusion The detection of AMA-M2 level plays an important role in the diagnosis and assessment of patient with PBC. It might contribute to the diagnosis of PBC patients with negative AMA-M2 in combination with the detection of anti-gp210,anti-SP100 and anti-PML antibodies.
出处
《广西医学》
CAS
2014年第11期1565-1567,共3页
Guangxi Medical Journal
基金
四川省卫生厅科研课题(130499)
关键词
胆汁性肝硬化
抗线粒体抗体
自身抗体
免疫功能
诊断
Primary biliary cirrhosis
Anti-mitochondrial antibody
Autoantibody
Immune function
Diagnosis
