摘要
目的:建立人肝癌细胞裸鼠移植瘤模型,并应用于苦参碱(matrine,Ma)的抑瘤作用观察.方法:将人肝癌细胞株Hep G2接种于BALB/c裸鼠腋部皮下,建立人肝癌细胞裸鼠皮下移植瘤动物模型.予Ma 100 mg/kg给药,随机分为给药3、2、1和0 wk(空白对照)组,均采用腹腔注射给药,每周连续给药5 d,期间观察裸鼠的全身状况、接种部位成瘤时间,测量动物体质量、瘤体体积和质量、计算抑瘤率以及采用HE染色法进行瘤组织的病理学观察,进而初步评价Ma的抗肿瘤作用.结果:将人肝癌细胞Hep G2种植于裸鼠腋部皮下造移植瘤模型成功,成瘤率达100%;观察发现M a对肝癌皮下移植瘤有抑制作用,给药1、2、3 wk组的抑瘤率分别为8.3%、37.5%、54%,给药3、2 w k组与0、1 w k组比较差异具有统计学意义(均P<0.01),给药3w k组与2 w k组比较、给药1 w k组与0 w k组比较差异也具统计学意义(P<0.05);HE染色给药组癌组织基本结构和癌细胞形态与对照组无明显变化,给药期间各组裸鼠的体质量等全身状况未出现明显降低且组间差异不明显.结论:人肝癌细胞裸鼠移植瘤模型建立成功,在肝癌模型中,Ma具有一定的抑瘤作用且无明显不良反应,早期给药比晚期给药抑瘤作用明显.
AIM： To establish a human hepatocellular carcinoma model in BALB/c nude mice and to assess the anticancer effects of matrine against human hepatocellular carcinoma. METHODS： Twenty-four 6-week-old male BALB/c nude mice were used in this study. A xenograft model of human hepatocellular carcinoma was established by subcutaneous injection of Hep G2 cells into nude mice. The nude mice were then randomly divided into 4 groups： a 3-wk group, a 2-wk group, a 1-wk group and a control group. Except for the control group, the mice in other groups were given matrine（100 mg/kg） by intraperitoneal injection for 5 d every week. The general status and body weight of mice, the time to tumor formation, tumor volume and weight, and the reduced rate of tumor growth were assessed, and pathological changes were observed after HE staining to evaluate the anticancer effect of matrine. RESULTS ： The success rate of establish-ing the xenograft model was 100%. Matrine had an inhibitory effect on the growth of tumor xenografts. The reduced rates of tumor growth was 8.3% in the 1-wk group, 37.5% in the 2-wk group, and 54% in the 3-wk group. The reduced rates of tumor growth were significantly higher in the 3-wk and 2-wk groups than in the 1-wk group and control group（P 〈 0.01）. There was also a significant difference between the 1-wk group and control group（P 〈 0.05）, and between the 3-wk group and 2-wk group（P 〈 0.05）. No obvious differences were found in histology of cancerous tissues between the three treatment groups and the con trol group. There were also no appar-ent differences in the general status or body weight of the animals among the four groups. CONCLUSION： A nude mouse xenograft modelof human hepatocellular carcinoma has been established successfully. Matrine at a dose of 100mg/kg has anticancer effects without obvious toxic effects.
出处
《世界华人消化杂志》
CAS
北大核心
2014年第33期5056-5062,共7页
World Chinese Journal of Digestology
基金
广西自然科学基金资助项目
Nos.桂财教2014GXNSFAA118143
桂科自0542119
广西高校优秀人才基金资助项目
No.桂教人20077029
广西卫生厅课题基金重点基金资助项目
No.桂卫重200887~~
关键词
肝细胞癌
动物模型
苦参碱
抗肿瘤
Hepatocellular carcinoma
Animal tumor model
Matrine
Anti-tumor
