摘要
目的观察清热消积方对肿瘤组织缺氧诱导转录因子-1α(HIF-1α)蛋白表达的影响,并探讨该方抗肿瘤血管生成的作用机制。方法将60只接种Lewis肺癌细胞株的C57BL/6J近交系小鼠随机分成空白对照组(NS组)、阳性对照组(CTX组)、清热消积方高、中、低剂量组(生药量1.12 g/m L、0.56 g/m L、0.28 g/m L),每组12只。空白对照组和清热消积方高、中、低3个剂量组按0.2 m L/10 g分别喂饲生理盐水和中药水煎液,阳性对照组腹腔注射环磷酰胺。用免疫组织化学法和免疫印迹法检测荷瘤小鼠肿瘤组织HIF-1α蛋白表达。结果清热消积方高、中、低剂量组、CTX组肿瘤组织HIF-1α蛋白的平均光密度(MOD)值均低于NS组(P<0.05),清热消积方高、低剂量组、CTX组肿瘤组织HIF-1α蛋白的阳性细胞所占值(A%)均低于NS组(P<0.05),清热消积方各剂量组间MOD值与A%均无差异(P>0.05)。结论清热消积方能下调荷瘤鼠肿瘤组织HIF-1α蛋白的表达,可能是其抗肿瘤血管生成的作用机制之一。
Objective To investigate the effect of Qing Re Xiao Ji on the expression of hypoxia inducing factor 1 alpha（HIF-1α）protein of Tumor Tissue,and to explore the mechanism of anti-tumor angiogenesis. Methods 60 C57BL/6J inbred mice with Lewis lung carcinoma xenograft model were randomly divided into five groups：blank control group（NS group）,positive control group（CTX group）and Qing Re Xiao Ji group divided into high,medium and low dose group（the amount of crude drug were 1.12g/m L,0.56g/m L, 0.28g/m L）, 12 rats in each group. The blank control group and Qing Re Xiao Ji high, medium, low dose group were respectively feed with physiological saline,traditional Chinese medicine decoction in 0.2m L/10 g,the positive control group was treated with intraperitoneal injection of cyclophosphamide. Using immunohistochemical method and Western blot to detect the expression of HIF-1α protein of Tumor Tissue. Results The MOD of HIF-1α protein in Qing Re Xiao Ji high,medium,low dose group,CTX group were lower than NS group（P〈0.05）,the A% of HIF-1α protein in Qing Re Xiao Ji high,low dose group,CTX group were lower than NS group（P〈0.05）,and Qing Re Xiao Ji in each dose group showed no difference between MOD and A%（P〉0.05）. Conclusion Qing Re Xiao Ji can cut the expression of HIF-1α protein,which may be one of anti-tumor angiogenesis effects.
出处
《云南中医学院学报》
2014年第6期13-16,共4页
Journal of Yunnan University of Traditional Chinese Medicine
基金
浙江省教育厅重点资助项目(Z2009-07128)
