摘要
目的：建立测定补肾强身片中金丝桃苷含量的方法。方法：采用反相高效液相色谱法。色谱柱为Amethyst C18-H,流动相为乙腈-0.1%磷酸溶液（16∶84,V/V）,流速为1.0 ml/min,柱温为30℃,检测波长为360 nm,进样量为10μl。结果：金丝桃苷的进样量在0.061 0~1.220 4μg范围内与其峰面积积分值呈良好的线性关系（r=0.999 9）;精密度、稳定性、重复性试验的RSD≤0.52%;平均加样回收率为98.68%,RSD为0.64%（n=6）。结论：本方法快速、简便,结果准确、可靠,可用于补肾强身片的含量测定。
OBJECTIVE： To establish a method for the content determination of hyperoside in Bushen qiangshen tablets. METHODS： RP-HPLC method was adopted. The determination was performed on Amethyst C18-H cohman with mobile phase consisted of acetonitrile-0.1% phosphoric acid solution （16：84, V/V） at the flow rate of 1.0 ml/min. The column temperature was 30 ℃, and the detection wavelength was set at 360 nm,the sample size was 10 μl. RESULTS： The linear range of hyperoside were 0.061 0-1.220 4 μg（r=0.999 9）with an average recovery of 98.68% （RSD=0.64% ,n=6）. RSDs of precision, reproducibility and stability tests were lower than 0.52%. CONCLUSIONS： This method is accurate, rapid, simple and reliable. It can be used for the content determination of Bushen qiangshen tablets.
出处
《中国药房》
CAS
北大核心
2015年第3期413-415,共3页
China Pharmacy
