摘要
目的临床研究微小RNA-96(MiR-96)、缺氧诱导因子-1α(HIF-1α)、Twist与Slug等易感基因在宫颈癌中的表达水平。方法选择2010年3月至2014年5月在该院就诊的宫颈癌患者128例为观察组,同时选择健康者100例作为对照组。测定MiR-96、HIF-1α、Twist与Slug等在宫颈癌组织中的表达水平。(1)HIF-1α:采用HIF-1α试剂盒对单克隆抗体HIF-1α进行检测,按照试剂盒的要求进行染色及制备组织标本,显微镜下观察阳性细胞率大于10%者为阳性;(2)MiR-96、Twist与Slug:用Trizol试剂按照试剂盒的说明提取总RNA,将RNA进行反转录,通过PCR定量检测MiR-96、Twist与Slug的相对表达值。结果(1)HIF-1α在正常组织中无阳性表达,而在肿瘤组织中阳性表达率远高于正常组织,两组比较差异有统计学意义(P<0.05);(2)MiR-96在肿瘤组织中的相对表达值远高于正常组织,两组比较差异有统计学意义(P<0.05);(3)Twist与Slug基因在肿瘤组织中的相对表达量远高于正常组织,两组比较差异有统计学意义(P<0.05)。结论与正常组织相比,MiR-96、HIF-1α、Twist与Slug基因在肿瘤组织中有显著的高表达。
Objective To investigate the expression levels of MiR‐96 ,HIF‐1α,Twist and Slug and other predisposing gene in cervical cancer .Methods In this study ,128 patients with cervical cancer treated in our hospital from March 2010 to May 2014 were selected as the observation group ,and 100 cases of healthy people were selected as control group .MiR‐96 ,HIF‐1α,Twist and Slug and other predisposing gene expression levels in cancer tissues were tested .（1） HIF‐1α：HIF‐1α kit was used to detect HIF‐1αmonoclonal antibodies ,the kit was prepared and stained according to the requirements ,and the positive cell rate greater than 10%under the microscope were positive .（2） MiR‐96 ,Twist and Slug：total RNA was extracted according to the instructions ,the RNA was reverse transcribed ,the relative expression values MiR‐96 ,Twist and Slug were detected by quantitative PCR .Results （1） HIF‐1αwas not expressed in normal tissues .And in tumor tissues ,the positive expression rate was much higher than that of normal tissue ,there was a significant difference（P〈0 .05）;（2）the relative expression values of MiR‐96 in tumor tissue were much greater than that of normal tissue （P〈0 .05）;（3） the relative expression rate of Twist and Slug gene in the tumor tissue were also much higher than that of normal organizations （P〈0 .05） .Conclusion Compared with normal tissues ,MiR‐96 ,HIF‐1α,Twist and Slug gene expression in tumor tissues are significantly greater .
出处
《重庆医学》
CAS
北大核心
2015年第3期340-342,共3页
Chongqing medicine
基金
江西省自然科学基金项目(2011ZBAB204018)
关键词
宫颈肿瘤
基因表达
疾病遗传易感性
表达水平
uterine cervical neoplasms gene expression genetic predisposition to disease expression levels
