摘要
建立了食品中柠檬黄、新红、苋菜红、胭脂红、日落黄、诱惑红和赤藓红7种合成色素的高效液相色谱分析法。样品经含有1%氨水的70%乙醇提取,聚酰胺粉末吸附、净化和富集后进行HPLC分析。采用Eclipse plus C18（250 mm×4.6 mn,5μm）色谱柱为分析柱,0.02 mmol/L乙酸铵水溶液-甲醇为流动相梯度洗脱,二极管阵检测器检测,外标法定量。结果表明：7种色素在0.5~20.0mg/L范围内有良好的线性关系,方法的检出限（S/N=3）分别为0.05、0.05、0.03、0.03、0.05、0.06和0.07 mg/kg,加标回收率为82.83%~105.39%,相对标准偏差不大于8.40%。
A method was established for the determination of 7 synthetic pigments in food by HPLC.The sample was extracted by the 70% ethanol containing 1% ammonia water,adsorbed by polyamide powder.The detection of 7 artificial synthetic pigments was carried out by HPLC using Eclipse plus C18（250 mm×4.6 mn,5μm）column as separation col-Hmn,0.02 mmol/L ammonium acetate solution-methanol as mobile phase.The target compounds were confirmed by diode-array detection and quantified by the external standard method.The results indicate that the calibration curves of thiophanate-methyl and carbendazim are linear in the range of 0.5~20.0mg/L with limits of detection of 0.05,0.05,0.03,0.03,0.05,0.06 and 0.07 mg/kg,respectively.The spiked recoveries of thiophanate-methyl and carbendazim are in the range of82.83%~105.39% with RSDs not more than 8.40%.
出处
《粮食与食品工业》
2014年第6期78-81,86,共5页
Cereal & Food Industry
