摘要
目的:优选益气固脱颗粒醇提工艺。方法:以正交设计的试验方法,对组方中有效成分黄芪甲苷、阿魏酸用HPLC法进行定量分析,选取L9(34)正交表,考察乙醇浓度、乙醇用量、提取次数、提取时间等工艺参数对益气固脱颗粒提取量的影响。结果:方差分析表明,乙醇浓度与提取次数对浸出物的含量及阿魏酸的提取有显著影响。最佳醇提工艺即加10倍量的70%的乙醇,提取3次,每次1.5 h。结论:该工艺稳定可行,适于工业生产。
Objective：To optimize the alcohol extraction process of Yiqigutuo granules. Methods： Using the L9 （34 ） orthogonal design method, the active ingredients astragaloside and ferulic acid in the formula were quantitatively analyzed by HPLC. The concen-tration and amount of ethanol, extraction times and extraction duration and the other parameters affecting the extraction volume were ex-amined. Results：Analysis of variance showed that the ethanol concentration and extraction times had significant influence on the ex-tract content and the extraction of ferulic acid. The best alcohol extraction process was as follows：70% ethanol of 10-fold volume, ex-tracting 3 times with 1. 5h for each time. Conclusion：The process is stable, and suitable for the industrial production.
出处
《中国药师》
CAS
2015年第3期410-413,共4页
China Pharmacist
基金
"十二五"南京市医学科技发展重大项目(编号:ZKX10027)
关键词
黄芪甲苷
阿魏酸
醇提工艺
正交试验
Astragaloside
Ferulic acid
Alcohol extraction process
Orthogonal test
