摘要
以二氯甲烷、N,N-二甲基甲酰胺为溶剂,以聚乳酸(PLA)为纺丝纤维基体,添加纳米氧化锌(Zn O)和表面活性剂吐温60,利用高压静电纺丝法,原位制备纳米Zn O/PLA复合纤维膜。所得纤维膜中,Zn O分散均匀,纤维平均直径约300 nm,且表面光滑。纺丝纤维的亲水性随着纳米Zn O的增加而大幅提高,断裂强度和延伸率随纳米Zn O含量的增加呈现先上升后下降的趋势。抗菌能力也呈现一定的Zn O含量依赖性。细胞毒性和细胞黏附实验表明,该抗菌纤维膜具有较好的细胞相容性。
The nano-zinc oxide（ Zn O,50nm） doped polylactic acid（ PLA） nanofibers were fabricated via insitu electrospinning technique. The mixture of dichloromethane（ DCM） and n,n-dimethyl formamide（ DMF） were pre-prepared for the solvent of PLA,then the nano-zinc oxide particles have been mixed into the solvents mixture.After optimazing the preparation procedure,the Zn O particles were well-distributed in the PLA solvents through observed by TEM. The structure of Zn O / PLA composite nanofibers has been observed by SEM,and the diameter was about 300 nm. Meanwhile,the so spun fibers were smooth and uniform without any bead-like structure The mechanical properties of composite nanofibers have been demonstrated by a tensile test,and the results demonstrated that the breaking strength and elongation rate were increased with the percentage of nano-Zn O. It showed that the addition of nano-Zn O may be an effective way to toughen the PLA nanofibers. Additionally,the hydrophilicity of the compound nanofibers has been recorded through a contact angle apparatus. It showed that the hydrophilicity of compound nanofibers were decreased monotonically with the increasing nano-Zn O content,obviously. Then,the cytotoxicity and the antibacterial properties have also been examined. These results suggested that the Zn O / PLA nanofibers were safe to cells and SEM observation also showed that cells had spread well onto the fiber mats with a high level of cell / scaffold interaction; this was demonstrated by the numerous pseudopodia and cell secretions that emerged. Finally,the ability to antibacterial was investigated through a Bacteriostatic ring experiment which showed that the antibacterial property had a Zn O-concentration dependent manner.
出处
《科学技术与工程》
北大核心
2015年第4期180-185,共6页
Science Technology and Engineering
基金
上海市自然科学基金(11ZR1435800)
国家自然科学基金(31100673)资助
