摘要
在流感疫情监测中,血凝试验是最常用的检测方法。针对鸡红细胞不能凝集流感病毒"O"相毒株,新鲜红细胞需现采现配、麻烦、费时,易溶血、不易保存等问题,本试验对豚鼠醛化红细胞制备方法的筛选和制备条件的优化进行研究,并以新鲜豚鼠红细胞为对照,用于流感病毒不同型别血凝试验。结果表明,戊二醛固定法制备的豚鼠醛化红细胞不溶血、结果稳定性好、保存期长、血凝活性与新鲜豚鼠红细胞基本一致,可以替代新鲜豚鼠红细胞用于血凝试验。
Hemagglutination test was a conventional detection method in epidemic surveillance of influenza.In order to overcome the defects of fresh red blood cells for troublesome and time-consuming preparation,easy hemolysis,short time conservation as well as chicken red blood cells could not agglutinate influenza virus＂O＂phase strains,our research optimized the method and the condition of preparation of hydorfomylated guinea pig red blood cells through HA test for different types of influenza virus and the fresh guinea pig red blood cells were taken as control.The results showed that guinea pig red blood cells hydorfomylated by glutaraldehyde were anhemolytic,and it had a good stability and a long time conservation.Compared with fresh guinea pig red blood cells,the effect of hemagglutination activity was almost identical.
出处
《中国畜牧兽医》
CAS
北大核心
2015年第3期569-574,共6页
China Animal Husbandry & Veterinary Medicine
基金
贵州省疾病预防控制中心科学技术基金(2013-E2-4)
关键词
流感病毒
醛化红细胞
血凝试验
influenza virus
hydroformylated red blood cells
hemagglutination test
