摘要
目的研究川芎嗪纳米制剂对大鼠腹膜间皮细胞的保护作用。方法胰酶消化法体外培养出大鼠腹膜间皮细胞(RPMCs),免疫组化进行鉴定;MTT法测定川芎嗪纳米喷雾剂对经TNF-α诱导大鼠腹膜间皮细胞的活力的影响。结果培养出的细胞经鉴定为RPMCs;经TNF-α诱导的RPMCs活力显著下降,而川芎嗪纳米制剂能够抑制经TNF-α诱导的RPMCs活力下降,对RPMCs有一定的保护作用。结论川芎嗪纳米制剂通过保护RPMCs而发挥其抗粘连作用。
【Objective】To study protective effect of tetramethylpyrazine nanoparticles on rat peritoneal mesothelial cells.【Methods】After enzyme digestion in vitro cultured rat peritoneal mesothelial cells（RPMCs） were identified by immunohistochemistry as RPMCs; MTT method was used to detect the effect of tetramethylpyrazine nanoparticles on the availability of TNF-α-induced rat peritoneal mesothelial cells.【Results】The cultured cells were identified as RPMCs; TNF-α-induced RPMCs activity significantly decreased, while tetramethylpyrazine could inhibit TNF-α-induced RPMCs activity decrease and had protective effect on RPMCs. 【Conclusion】Tetramethylpyrazine nanoparticles exert its anti-adhesion effect via protecting RPMCs.
出处
《中国现代医学杂志》
CAS
北大核心
2015年第7期24-27,共4页
China Journal of Modern Medicine
基金
江苏省中医药局科技项目(No:LZ13006)
南京中医药大学青年自然科学基金(No:13XZR09)
关键词
川芎嗪
纳米制剂
腹膜间皮细胞
原代细胞
腹腔粘连
tetramethylpyrazine
nanoparticles
peritoneal mesothelium cells
primary cells
abdominal adhesions
