摘要
研究富硒玉米肽对四氯化碳(Carbon tetrachloride,CCl4)诱导的小鼠肝损伤的保护作用。将健康的雄性昆明小鼠70只,随机分成7组:正常对照组、CCl4模型组、富硒玉米肽低、中、高剂量组(100、200、400mg/kg·bw)、普通玉米肽组(200 mg/kg·bw)、水飞蓟素阳性对照组(50 mg/kg·bw),每组10只。测定比较血清中谷丙转氨酶(ALT)活力、谷胱甘肽(GSH)、三酰甘油(TG)含量,同时测定肝组织中丙二醛(MDA)、谷胱甘肽(GSH)的含量及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力,肝脏指数,并观察肝组织病理学变化。结果显示富硒玉米肽低、中、高剂量组均能极显著抑制CCl4致肝损伤小鼠血清ALT活性和TG含量的升高、GSH含量的降低(P<0.01);均能极显著提高肝组织中GSH含量和SOD、GSH-Px活力,并降低肝组织中MDA的含量(P<0.01),极显著降低由于肝损伤引起的肝脏肿大(P<0.01),改善肝组织损伤程度,并存在剂量效应关系。富硒玉米肽高剂量组的护肝效果与50 mg/kg·bw的水飞蓟素相当,相同剂量的富硒玉米肽的保肝作用极显著优于普通玉米肽(P<0.01)。结果表明富硒玉米肽对CCl4所致小鼠急性肝损伤有显著的保护作用,且与普通玉米肽相比,富硒玉米肽的有效保肝剂量显著降低。
The hepatoprotective effects of the selenium- enriched corn peptides have been evaluated to treat carbon tetrachloride（ CCl4）- induced liver injury in mice. Seventy healthy Kunming male mice were randomized into 7 groups for 10 mice / group as normal control group,CCl4- injured model group,low dose group,medium dose group and high dose group（ 100,200 and 400 mg / kg·bw respectively）,selenium- enriched corn peptides treatment group, corn peptides（ 200 mg / kg · bw） treatment group and positive control group- silymarin（ 50 mg / kg·bw） treatment group. The activity of alanine aminotransferase（ ALT）,the glutathione（ GSH） level,the triglyceride（ TG） level in serum,the activity of superoxide dismutase（ SOD） and glutathione peroxidase（ GSH-Px）,malondialdehyde（ MDA） level and glutathione（ GSH） level in liver were determined as well as the hepatic tissue index. The morphology of hepatic tissue was also examined. The results showed that selenium- enriched corn peptides could significantly decrease ALT＇Perilla Ls activity,TG content level in serum,MDA level in liver（ P〈0. 01） and the liver enlargement. The selenium- enriched corn peptides could significantly increase GSH level,SOD activity,GSH- Px activity in liver（ P〈0. 01） in a dose- dependent manner. The biochemical results were supplemented by histopathological examination of liver section. The hepatoprotective effect of selenium- enriched corn peptides was significantly better than the normal corn peptides with the same dose（ P〈0. 01）. When selenium- enriched corn peptides concentration reached to 400 mg / kg·bw,its effect was approximate to silymarin（ 50 mg / kg·bw）. In conclusion,the study demonstrates that selenium- enriched corn peptides had a significant protective effect against CCl4- induced liver damage. The dose of selenium- enriched corn peptides with the potent hepatoprotective effect was significantly less than that of the normal corn peptides.
出处
《中国粮油学报》
EI
CAS
CSCD
北大核心
2015年第3期6-11,共6页
Journal of the Chinese Cereals and Oils Association
基金
国家自然科学基金(30972043)
863计划(2008AA10Z314)
中央高校基本科研业务费专项(2013PY095)
关键词
富硒玉米肽
CCl4肝损伤模型
保肝
selenium-enriched corn peptides
CCl4-induced liver injury model
hepatoprotection
