摘要
光系统Ⅱ(PSⅡ)利用锰簇(OEC)氧化水分解并将电子通过类囊体膜上的电子传递链传递到外源电子受体侧.但是去除锰簇的光系统Ⅱ(Tris-Wash BBY)是否具有电子传递能力仍是一个值得研究的问题.因此本文引进外源电子供体1,5-二苯基卡巴肼(DPC)代替锰簇向电子受体侧提供电子,研究Tris-Wash BBY的电子转移活性.实验表明Tris-Wash BBY在光照的条件下可以将DPC的电子传递给外源电子受体2,6-二氯酚靛酚(DCPIP),并且利用紫外可见光谱的方法,通过分析外源电子受体DCPIP的还原量来表征Tris-Wash BBY和BBY的电子传递活性.
Photosystem Ⅱ （BBY） could split water on manganese cluster （OEC） and transfer electrons to exogenous electron acceptor side through the thylakoid membrane electron transport chain. But it is still worthy to study whether photosystem Ⅱ（Tris-Wash BBY） which removed the manganese cluster has capability of the electron transfer. Therefore, this article introduces an exogenous electron donor 1,5-Diphenylcarbazide （DPC） insteading of manganese cluster to provide electron for measuring the activity of electron transfer of Tris-Wash BBY. The results show that electrons could transport from DPC to DCPIP under illumination in Tris-Wash BBY. We could characterize electron transfer activity of Tris-Wash BBY and BBY by analyzing the reduction amount of DCPIP based on UV-visible spectroscopy methods.
出处
《辽宁师范大学学报（自然科学版）》
CAS
2015年第1期77-83,共7页
Journal of Liaoning Normal University:Natural Science Edition
基金
国家自然科学基金项目(31100618)
