摘要
目的观察Cdk5抑制短肽(TFP5)对2型糖尿病小鼠模型体重和血糖的干预作用。方法早期干预:分为正常小鼠生理盐水组(1 m L·只-1,组1),正常小鼠注射TFP5组(200 mmol,1 m L·只-1,组2),db/db小鼠注射生理盐水组(1 m L·只-1,组3),db/db小鼠注射TFP5(200 mmol,1 m L·只-1,组4)。从第5周开始每日注射1次,以后均为隔日注射1次,注射至第8周,共注射3周,第8周时处死小鼠。晚期干预:分db/db小鼠注射生理盐水组(组5)和db/db小鼠注射TFP5(400 mmol,组6),从第15周开始每日注射1次,第16周改为隔日1次,第17周停止注射1周,18周处死。注射方式均为腹腔注射,由第5周时体重每周测2次,处死之前测体重,取血送血糖监测。结果 2组正常小鼠的血糖和体重差异均无统计学意义(P<0.05);db/db小鼠注射生理盐水组血糖明显高于注射TFP5组(P<0.05),6周后db/db小鼠注射生理盐水组体重明显高于注射TFP5组(P<0.05);2组在第18周时,体重及血糖均差异无统计学意义(P>0.05)。结论应用TFP5能够降低T2DM模型小鼠的血糖及体重,但其治疗效果可能受到治疗时机、疗程的长度等的影响。
Objective To study and observe TFP5 on weight and blood glucose in T2 DM animal models. Methods Experiment1（ early intervention） ： group 1 was not treated control group of normal mice,and group 2 of mice injected TFP5 the normal group,the third group of db / db mice injected with saline（ 1 ml）,and group 4 of db / db mice injected TFP5（ 200 mmol,1ml）. From the beginning of the first five weeks to eight weeks for injection,injected once a day the first week,after both injected once every other day of injection three weeks,the mice were sacrificed 8 weeks. Experiment 2（ late intervention）,a group of db / db mice were injected with saline group（ Group 5）,another group of db / db mice injected TFP5（ 400 mmol,group 6）. Injection time is to start from the first 15 weeks,daily injections once every other day the second week of injection two weeks,stop the injection a week,18 weeks to death. Mice were injected intraperitoneally way,weight measured twice weekly until death measure weight,blood biochemical tests sent. Results The blood glucose was significantly higher in group 3 than that in group 4（ P 〈 0. 05）; 6 weeks after group 3 was significantly higher than the weight of the group 4,and the gap between the two weight increase with time（ P 〈 0. 05）; Between group 5 and 6,body weight and blood glucose were not significantly different at all in the 18 week（ P 〉 0. 05）. Conclusion Application TFP5 confirmed T2 DM can improve islet function in mice with reduced blood glucose and weight; its therapeutic effect may be the timing of treatment,length of treatment of other effects.
出处
《宁夏医学杂志》
CAS
2015年第3期200-202,共3页
Ningxia Medical Journal
基金
国家自然科学基金资助项目(NSFC81160093
NSFC81460161)
宁夏自然科学基金资助项目(NZ14160)
宁夏科技攻关资助项目(KGX1910-16)
宁夏科技攻关国际合作项目(2011ZYH169)
宁夏科技支撑资助项目(2013ZY503)
