摘要
以草地早熟禾‘午夜2号’为材料,采用高效液相色谱法(HLPC),测定了不同施肥处理下草地早熟禾的GA3含量,着重分析了GA3含量的变化与其生长的相关性.并以研究结果为依据筛选出最优的施肥组合.结果表明:施用6.52g二铵+7.18g尿素+30g菌肥+1.5g柠檬酸(处理5),草地早熟禾地上部分生长状况优良,叶片质地最为纤细,为2.13mm,叶绿素含量最高为3.10μg/g,最大密度为123.11株/m2,最大盖度为98.20%,高度可达19.37cm.且根系生长旺盛,根长最长,为7.43cm,最小根冠比为2.26,根体积可达0.058 8cm3,根系活力高达200.99μg/(g·h).说明:适宜的内源激素水平决定着植株体生长发育的状态,不同施肥处理下草地早熟禾各部位在不同生长时期内GA3含量随着施肥时间的增长而上升.在处理5下,草地早熟禾GA3地上、地下部分平均含量最高,分别为537.25ng/g和478.45ng/g.研究结果还证实了所采用的测定方法简便易行,精密度高,重现性好.
The kentucky bluegrass （Midnight Ⅱ,Baron,Kentucky）were used as material.GA3 content was determined by high performance liquid chromatography （HPLC）,the relationship was analyzed on the growth of plant and the changes of GA3 content,and the fertilizer combination was optimized.The results showed that the turf quality was significantly improved by using the fertilizer combination with 6 .5 2 g diamine＋7.18 g urea＋30 g bacterial manure＋1.5 g citrate （treatment 5）.The texture of leaf was the smoothest with 2.13 mm,the chlorophyll content was 3.10 mg/g,the density was 123.11 plants/m^2 ,cover degree was 98.20% and the maximum height was 19.37 cm.Meanwhile,roots had most vigorous growth after above fertilizer treatments.The root length was 7.43 cm,the maximum root cap ratio was 2.26,root volume was 0.058 8 cm^3 and root activity was 200.99 mg/（g·h）.GA3 contents of roots,stems and leaves were reached the highest under the fertilizer treatments.GA3 content of aboveground and underground was 537.25 ng/g and 478.45 ng/g under treatment 5.This study also confirmed that the methods was simple, high precision and good reproducibility.
出处
《甘肃农业大学学报》
CAS
CSCD
北大核心
2015年第1期103-109,115,共8页
Journal of Gansu Agricultural University
基金
国家自然科学基金项目"草地早熟禾种间体细胞杂交的研究"(31160482)
关键词
草地早熟禾
施肥
GA3
生长发育
Kentucky bluegrass
fertilizer
growth and development
