摘要
目的:研究大黄酰缬氨酸对人宫颈癌Hela细胞生长的抑制作用和放射增敏活性。方法:不同浓度大黄酰缬氨酸(0、6.03、18.09、54.28、162.85、488.56、732.84、1 465.68μmol/L)作用于Hela细胞24、48 h后采用MTT法测定细胞活力,计算抑制率、半数抑制浓度(IC50)与20%抑制浓度(IC20)并绘制浓度-抑制率曲线。试验分为模型(等容培养液)6组与大黄酰缬氨酸(30μmol/L)6组,培养细胞24 h后分别接受0、1、2、4、6、8 Gyγ射线照射(剂量率:0.78 Gy/min),再继续培养24 h,绘制照射剂量-存活比曲线,计算N(外推数)、D0(平均致死剂量)、SF2(单次照射2 Gy的细胞存活比)、SER(模型组D0与用药组D0之比或模型组SF2与用药组SF2之比)。结果:54.28、162.85、488.56、732.84、1 465.68μmol/L大黄酰缬氨酸作用24 h时和6.03、18.09、54.28、162.85、488.56、732.84、1 465.68μmol/L大黄酰缬氨酸作用48 h时均可明显抑制细胞活力;其对宫颈癌He La细胞的抑制作用呈剂量依赖性,IC50分别为179.423μmol/L(24 h)和84.192μmol/L(48 h),IC20分别为52.943μmol/L(24 h)和30.505μmol/L(48 h)。与模型组比较,大黄酰缬氨酸组细胞N、D0、SF2降低,SER升高。结论:大黄酰缬氨酸体外能明显抑制Hela细胞生长,增加Hela细胞的放射敏感性。
OBJECTIVE：To investigate the growth inhibition effect and radiosensitization effect of acyl-valine from Rheum palmatum on human cervical cancer Hela cells. METHODS：MTT method was used to determine cell viability after the acyl-valine from R. palmatum varying in concentration（0,6.03,18.09,54.28,162.85,488.56,732.84 and 1 465.68 μmol/L）had acted on Hela cells for 24 h and 48 h. The inhibition rate and median inhibitory concentration（IC50）and twenty percent inhibitory concentration（IC20）were calculated. The concentration-inhibition rate curves were drawn. In the experiment,there were 6 model groups（isovolumic culture solutions） and 6 groups of acyl-valine from R. palmatum（30 μmol/L）. After being cultured for 24 h,the cells were irradiated with γ-ray at doses of 0,1,2,4,6 and 8 Gy（dose rate：0.78 Gy/min）,followed by culture for another 24 h.Then the dose-survival ratio curves were drawn to get N（extrapolation number）,D0（mean lethal dose）,SF2（survival ratio after single irradiation at a dose of 2 Gy） and SER（ratio of D0 of model group to that of medication group or ratio of SF2 of model group to that of medication group）. RESULTS：The acyl-valine from R. palmatum with the concentration of 54.28,162.85,488.56,732.84 and 1 465.68 μmol/L after 24 h and that with the concentration of 6.03,18.09,54.28,162.85,488.56,732.84,1 465.68 μmol/L after 48 h could significantly inhibit the cell viability. The inhibition effect of acyl-valine from R. palmatum on cervical cancer Hela cells was manifested in a dose-dependent manner. IC50 was 179.423 mol/L（24 h）and 84.192 mol/L（48 h）,IC20 was 52.943 μmol/L（24 h）and 30.505 μmol/L（48 h）,respectively. Compared with model group,N,D0 and SF2were decreased and SER was increased. CONCLUSIONS：The acyl-valine from R. palmatum can significantly inhibit the growth of cervical cancer Hela cells in vitro and increase the radiosensitivity.
出处
《中国药房》
CAS
北大核心
2015年第10期1360-1362,共3页
China Pharmacy
关键词
大黄酰缬氨酸
人宫颈癌细胞
放射增敏
Acyl-valine from Rheum palmatum
Human cervical cancer Hela cells
Radiosensitization
