摘要
目的研究骨桥蛋白基因单核苷酸多态性及其单倍型与广西壮族人群鼻咽癌易感性的关系。方法采用病例对照研究方法，收集2010年3月1日至2013年3月20日入院的广西壮族鼻咽癌患者，同时直接抽样法选取2012年1月1日至8月1日常规体检的广西壮族健康人群作为对照。利用单碱基延伸PCR技术和DNA测序法，检测150例广西壮族鼻咽癌患者和150名壮族对照者的骨桥蛋白基因rs1126772和rs9138位点单核苷酸多态性，再用×。检验比较基因型和等位基因型分布频率在鼻咽癌组和对照组之间的差异，并分析骨桥蛋白基因的单倍型频率。结果骨桥蛋白基因位点rs1126772在病例组和对照组问的基因型和等位基因型频率分布差异无统计学意义（GA／GG：OR=0．94，95％C10．37～2．37，X2=0．182，P=0．891；AA／GG：OR=0．86，95％C10．35～2．12，X2=0．834，P=0．773）。rs9138位点在病例组和对照组间的基因型和等位基因型频率差异也无统计学意义（CA／CC：OR=1．42，95％C10．88～2．29，x2=2．023，P=0．155；AA／CC：pR=1．77，95％C10．78～4．01，X2=1．901，P=0．168）。但是，单倍型分析结果显示，病例组GA单倍型频率显著高于对照组（P=0．003），GA单倍型携带者罹患鼻咽癌的风险显著增加（OR=4．84，95％CI1．59～14．71）。结论在广西壮族人群中，骨桥蛋白基因位点rs1126772和rs9138的GA单倍型增加个体鼻咽癌的易感性。
Objective To evaluate the relationship between osteopontin gene genetic polymorphisms and susceptibility of nasopharyngeal carcinoma in Guangxi Zhuang people. Methods With a hospital based case-control study, osteopontin gene polymorphisms were compared between patients with nasopharyngeal carcinoma and healthy outpatients as a controls in Zhuang population in Guangxi. The single nueleotide polymorphisms at rs1126772 and rs9138 sites of the osteopontin gene were determined by polymerase chain reaction-single base extension technique （PCR-SBE） and DNA sequencing technology. The comparison between genotype and allele frequency distribution differences in case and control group was accomplished by a X2 test. The frequencies of haplotypes in osteopontin gene in different groups were analyzed. Results There were no differences between the patients and controls in the genotype or allele frequencies of osteopontin gene rs1126772 site（GA/GG：OR =0. 94, 95% CI 0. 37 -2.37, x2 =0. 182,P =0. 891 ; AA/ GG：OR =0. 86, 95%CI0. 35 -2. 12,X2 =0. 834, P =0. 773） or rs9138 site（CA/CC：OR = 1.42, 95%CI 0.88 -2.29, x2 =2.023, P =0.155; AA/CC：OR = 1.77, 95%C10.78 -4.01, x2 = 1.901, P = 0. 168）. The frequency of GA haplotype in the patients was significantly higher than that in the controls （P = 0. 003 ）, and the GA haplotype was associated with a significantly increased risk of nasopharyngeal carcinoma （ OR = 4. 84, 95% CI 1.59 - 14. 71 ）. Conclusion The haplotype GA of osteopontin gene rs1126772 and rs9138 sites increases the risk of nasopharyngeal carcinoma in Guangxi Zhuang people.
出处
《中华耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2015年第2期127-130,共4页
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
基金
广西自然科学基金（2012GXSFBA053121）
百色市科学研究与技术开发项目（百科计20140617）
关键词
骨桥蛋白
多态性
单核苷酸
鼻咽肿瘤
单倍性
Osteopontin
Polymorphism, single nucleotide
Nasopharyngeal neoplasms
Haploidy
