摘要
目的 分析溃疡性结肠炎（UC）与血清氨基酸水平的联系,从代谢组学角度探讨UC病因及发病机制,寻找用于早期诊断及指导治疗的新型生物标志物.方法 采集2012年10月至2013年11月解放军第三○九医院住院治疗的44例UC患者及同期52例健康体检者的血清样本及临床资料.通过高效液相色谱-串联质谱联用技术（HPLC-MS/MS）稳定同位素内标法对UC患者血清中氨基酸代谢轮廓进行分析.结果 UC患者血清中谷氨酸、谷氨酰胺、甲硫氨酸、色氨酸和组氨酸水平明显低于正常对照组[（59.20 ±21.93） μmol/L比（88.14 ±34.85） μmol/L;（2 200.51 ±648.03） μmol/L比（2 664.91±1 034.74） μmol/L; （268.69±211.64） μmol/L比（431.48±298.00） μmol/L;（68.83±32.33） μmol/L比（89.96 ± 29.29） μmol/L;（101.88±32.01）μmol/L比（115.10±17.84） μmol/L;P均＜0.05],天冬酰胺和异亮氨酸水平明显高于正常对照组[（195.14±122.14） μmol/L比（140.49±34.91）μmol/L;（94.61 ± 29.76） μmol/L比（80.99±19.73） μmol/L;P均＜0.05].结论 UC患者血清中多种氨基酸含量的异常提示UC存在氨基酸代谢状态的改变,某些氨基酸与UC的发病及进展存在联系,有可能成为潜在的UC生物标志物.
Objective To determine the relationship between serum amino acid composition and pathogenesis of ulcerative colitis （UC）,so as to explore some new biomarkers for early diagnosis and treatment.Methods This study was conducted in the 309th Hospital of Chinese People＇s Liberation Army between October 2012 and November 2013.Forty-four UC patients and 52 healthy controls were enrolled and the serum samples were collected.Using high performance liquid chromatography-mass spectrometry technology （HPLC-MS/MS） stable isotope internal standard method,we detected serum amino acid metabolic profiling in the two groups.Results Compared with the control group,the serum levels of glutamic acid,glutamine,methionine,tryptophan and histidine in UC group were significantly lower [（59.20 ±21.93） μmol/L vs （88.14 ±34.85） μmol/L; （2 200.51 ±648.03） μmol/L vs （2 664.91 ±1 034.74）μmol/L;（268.69 ±211.64） μmol/L vs （431.48 ±298.00） μrmol/L; （68.83 ±32.33） μmol/L vs （89.96 ± 29.29） μmol/L; （101.88 ± 32.01） μmol/L vs （115.10 ± 17.84） μmol/L respectively,all P 〈0.05].However,the serum levels of asparagine and isoleucine in UC group were significantly higher than the control group[（195.14 ± 122.14） μmol/L vs （140.49 ± 34.91） μmol/L; （94.61 ± 29.76） μmol/L vs （80.99 ± 19.73） μmol/L respectively; both P 〈 0.05].Conclusion The amino acid metabolic profiling in UC patients is different from that in healthy controls,which suggests that there might be a certain relationship between amino acid composition and pathogenesis of UC.Some amino acids would be tested as potential biomarkers in patients with UC.
出处
《中华内科杂志》
CAS
CSCD
北大核心
2015年第3期210-213,共4页
Chinese Journal of Internal Medicine
关键词
结肠炎
溃疡性
氨基酸类
代谢组
高效液相色谱-质谱联用技术
稳定同位素内标法
Colitis,ulcerative
Amino acids
Metabolome
High performance liquid chromatography-mass spectrometry technology
Stable isotope internal standard method
