摘要
目的 分析不同临床阶段前列腺癌和BPH患者血清中差异性表达的微小RNA（microRNA,miRNA）,筛选与前列腺癌相关的miRNA. 方法 选取2008年9月至2013年11月收治的前列腺癌患者血清46例,包括激素依赖性前列腺癌（androgen-dependent prostate cancer,ADPC）和激素非依赖性前列腺癌（androgen-independent prostate cancer,AIPC）患者血清各23例,选取23例BPH患者的血清作为对照组.所有前列腺癌患者均经前列腺穿刺活检获得病理学诊断,BPH患者经TURP术后病理确诊.各组随机选取3例共9例血清进行miRNA基因芯片分析,其余60例样本进行实时qPCR对部分差异表达的miRNA进行验证. 结果 对ADPC、AIPC和BPH组各3例血清进行miRNA芯片分析,共筛选出31个差异表达的miRNA.ADPC组与BPH组比较有8个miRNA表达存在差异,其中7个miRNA表达上调,1个miRNA表达下调;AIPC组与BPH组比较有9个miRNA表达上调,6个miRNA表达下调;miR-181a-2在ADPC组和AIPC组中均明显高表达.AIPC组与ADPC组比较各有4个miRNA表达上调和下调.实时qPCR验证了miR-29a、miR-181a-2、let-7b、miR-144、miR-134、miR-15b、miR-491-3p、miR-495等在各组血清中的差异表达与芯片检测结果一致. 结论 血清中差异性表达的miRNA可能成为潜在的前列腺癌诊断和治疗靶点,其中miR-181a-2可能与前列腺癌的发生发展有关.
Objective To investigate the differentially expressed microRNA （miRNA） in serums of benign prostatic hyperplasia （BPH） patients and prostate cancer patients in different clinical stages by using miRNA microarray and confirm the relationship between those miRNAs and prostate cancer.Methods Serums from 23 patients with BPH and 46 patients with prostate cancer,including 23 cases with androgen-dependent prostate cancer and 23 cases with androgen-independent prostate cancer,were collected from Sep.2008 to Nov.2013.Cases with BPH were pathologically diagnosed after TURP,and cases with prostate cancer were confirmed by prostate needle biopsy.3 samples were randomly selected from each group,and a total of 9 samples were used for determining miRNA expression by miRNA microarray chip.Data analysis was performed to select significant difference of miRNA expression profile in prostate cancer,and the difference was confirmed by quantitative real-time PCR analysis on another 60 samples.Results 9 serum samples （3 cases of ADPC,3 cases of AIPC and 3 cases of BPH） were carried out miRNA microarray analysis and screened 31 differentially expressed miRNAs in three groups.8 aberrant miRNAs were detected in ADPC group in comparison to BPH group,including 7 upregulated and 1 downregulated miRNAs.9 upregulated and 6 downregulated miRNAs were detected in AIPC group in comparison to BPH group.Particularly,miR-181a-2 was simultaneously aberrantly expressed in ADPC group and AIPC group.In addition,4 upregulated and 4 downregulated miRNAs were detected in AIPC group in comparison to ADPC group.Ultimately,the different expression levels of miR-29a,miR-181a-2,let-7b,miR-l4,miR-134,miR-15b,miR-491-3p,and miR495 were confirmed by Real-time qPCR,suggesting the high reliability of miRNA array.Conclusions The differentially expressed miRNA in serum might have diagnostic potential and be seen as therapeutic targets in human prostate cancer.Interesting,miR-181a-2 might anticipate in the progression of prostate cancer.
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
2015年第3期225-228,共4页
Chinese Journal of Urology
基金
国家自然科学基金（81172706）
