摘要
目的:分离、培养人脐带基质来源的间充质干细胞(h UC-MSCs),观察其部分肝细胞生物学特性。方法:利用组织块贴壁培养法从脐带基质中分离培养h UC-MSCs,观察细胞形态,采用细胞免疫荧光法检测表面标记物CD44,通过RT-PCR法检测肝细胞标志基因ALB、CK18、G6P、GLUL、MET、TAT的表达情况,并进行PAS糖原染色。结果:从脐带基质分离出成纤维样细胞形态的h UC-MSCs,CD44表达阳性;RT-PCR结果表明培养的h UC-MSCs表达成熟肝细胞的标志基因ALB、CK18、G6P、GLUL和MET,而不表达TAT,PAS糖原染色呈阳性反应。结论:组织块贴壁培养法可有效获得稳定、均质性良好的h UC-MSCs,其具备部分肝细胞生物学特性。
Aim ： To investigate the expressions of p53 and p21waf/cipl in blood of children with idiopathic short stature（ISS） and to elucidate the pathogenesis of ISS. Methods： Real-time PCR was used to measure the expressions of p53 mRNA and p21waf/cipl mRNA in peripheral blood from 30 children with ISS（ISS group） and 30 normal control（ control group） ; ELISA was used to measure the levels of P53 and P21waf/cipl in serum of the children in the 2 groups. Results： The expression of p53 mRNA and the concentration of serum P53 had no statistical significance between the 2 groups（ t = 1. 044 and 0. 701,P 〉 0.05）. The expression of p21 waf/cipl mRNA in ISS group was significantly higher than those in the control group（ t = 4. 066 ,P 〈 0. 001 ）. Conclusion ： p21waf/cipl may be responsible for the pathogenesis of ISS, which might not depends on p53 pathway.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2015年第2期187-191,共5页
Journal of Zhengzhou University(Medical Sciences)
基金
河南省基础与前沿技术研究项目资助092300410015,082300450560
关键词
脐带
间充质干细胞
肝细胞生物学特性
umbilical cord
mesenchymal stem cell
hepatocyte biological characteristic
