摘要
五没食子酰葡萄糖(1,2,3,4,6-五-O-没食子酰葡萄糖,PGG)是桂枝茯苓胶囊中的一个主要活性成分,具有多种生物学与药理学活性,但其传统的分离纯化工艺较复杂,效率低,溶剂用量大,污染环境,而分子印迹技术(molecular imprinting technique,MIT)是近年来发展起来的一种新的分子识别技术,其制备的分子印迹聚合物具有特异性结合位点,对目标分子的分离有较高的选择吸附性,能克服以上传统分离技术的缺点。该研究以PGG为模板,采用分子印迹技术制备PGG表面分子印迹聚合物(surface molecularly imprinted polymer,SMIP),并对其吸附性能进行了研究,同时以该聚合物为填料自制色谱柱,从桂枝茯苓胶囊甲醇提取物中分离制备PGG,经HPLC检测质量分数达90.2%。该方法可用于从桂枝茯苓胶囊复方中快速大量制备PGG,有利于减少中药复方分离过程中有机溶剂的使用,环境友好,且操作简便,为中药及复方活性成分的分离纯化提供了一种新的思路和方法。
1,2,3,4,6-penta-O-galloyl-D-glucose（ PGG） is one of the main active compounds of Guizhi Fuling capsule. Molecularly imprinted polymers（ MIP） have high affinity toward template molecules synthesized by molecularly imprinted technology for its specific combined sites,which can overcome the shortcoming of traditional separation methods,such as complex operation,low efficiency,using large quantity of solvent and environmental pollution. In this paper,surface molecularly imprinted polymer（ SMIP） was prepared by surface imprinting with PGG as the template molecule. Its adsorption capacity was measured by the scatchard equation. The separation of PGG from Guizhi Fuling capsule at preparatived scale was achieved with molecularly imprinted polymer as stationary phase and the purity was 90. 2% by HPLC. This method can be used to prepare PGG from Guizhi Fuling capsule with large capacity and is easy to operate. It provides a new method for efficient separation and purification for other natural products.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2015年第6期1012-1016,共5页
China Journal of Chinese Materia Medica
基金
国家"重大新药创制"科技重大专项(2013ZX09402203)
关键词
分子印迹技术
PGG
桂枝茯苓胶囊
表面分子印迹聚合物
molecularly imprinted technology
PGG
Guizhi Fuling capsule
surface molecularly imprinted polymer
