摘要
目的观察大黄鞣质对SD大鼠创伤性脑损伤继发脑水肿的抑制作用并探讨其作用机制。方法采用Feeney自由落体坠击法制备大鼠创伤性脑损伤模型,腹腔注射大黄鞣质,测定给药后大鼠脑组织含水量、血管通透性及SOD水平的变化,采用免疫组织化学法和蛋白质免疫印迹法测定大鼠脑组织AQP4和GFAP表达的变化。结果与模型组相比,给药组大鼠脑组织含水量显著下降(P<0.05),脑组织SOD水平显著提高(P<0.05),血管通透性明显下降(P<0.05);免疫组化法检测给药组大鼠AQP4和GFAP的阳性细胞数减少,颜色变浅,阳性细胞表达评分明显低于模型组各亚组(P<0.01);蛋白印迹免疫试验法检测给药组AQP4和GFAP的阳性表达下降,其表达量与模型组相比,差异有统计学意义(P<0.01)。结论大黄鞣质对大鼠创伤性脑损伤继发脑水肿的抑制作用与降低血管通透性、增加SOD水平和降低水通道蛋白AQP4和GFAP的表达有关。
Objective： To observe the influence of Rhubarb tannins on encephaledema followed by traumatic brain injury in SD rats.Methods： In order to prepare of traumatic brain injury model,the classic Feeney free fall drop was used to wounded rats.Rhubarb tannins were given rats by intraperitoneal injection.The brain of rat water content,cerebral vascular permeability,SOD level,cell AQP4 and GFAP expression using immune histochemical method and protein determination of western blot test were determination.Results： The brain of rat water content and the cerebral vascular permeability in the Rhubarb tannins group were significantly lower than those in the model group（P〈0.05）.The SOD level in Rhubarb tannins group was higher obviously than that in the model group（P〈0.05）.The AQP4 and the GFAP in Rhubarb tannins group were decreased remarkably.There were some statistical differences between the Rhubarb tannins group and the model group（P〈0.05）.Conclusion：Rhubarb tannins can cure encephaledema in rats followed by traumatic brain injury.Its mechanism is through reduction of cell AQP4 and GFAP expression,decrease of cerebral vascular permeability and increase of SOD level to achieve protective effect of encephaledema.
出处
《中国中医急症》
2015年第3期380-383,共4页
Journal of Emergency in Traditional Chinese Medicine
基金
卫生部国家临床重点专科建设项目
关键词
大黄鞣质
脑损伤
脑水肿
抑制作用
Rhubarb tannins
Brain injury
Encephaledema
Inhibition
