摘要
目的观察五味子提取物对妊娠前苯并[a]芘(Ba P)暴露致早孕期大鼠胚胎损伤的防治作用,并探讨其内在机制。方法将75只♀SD大鼠随机分为5组:正常对照组、Ba P模型组、五味子低、中、高剂量组,每组15只。给药15 d后制备孕鼠模型,于妊娠d 9处死孕鼠,记录孕鼠各期体质量、子宫连胚总质量,并计算脏器系数;ELISA试剂盒检测大鼠血清β-CG及PROG水平;荧光定量PCR检测孕鼠胚胎中Bax、Bcl-2和caspase-3 mRNA表达水平;Western blot检测孕鼠胚胎中Bax、Bcl-2和caspase-3蛋白表达水平。结果 1与对照组相比,模型组大鼠体质量、子宫连胚总系数、大鼠血清β-CG及PROG水平均下降,Bax及caspase-3 mRNA水平及蛋白表达上调,Bcl-2 mRNA水平及蛋白表达下调;2五味子提取物治疗后,孕鼠体质量、子宫连胚总系数、大鼠血清β-CG及PROG水平较模型组上升,Bax及caspase-3 mRNA水平及蛋白表达下调,Bcl-2 mRNA水平及蛋白表达上调。结论妊娠前苯并[a]芘暴露可对早孕期大鼠产生胚胎毒性,而五味子提取物可以抑制Ba P造成的生殖损伤,这一保护作用可能是通过调节凋亡相关因子来实现的。
Aim To investigate the role of Schisandra chinesis extract （ SCE ） in benzo [ a ] pyrene （ BaP ）-in-duced apoptosis in embryo of rat and its underlying mechanism. Methods 75 SD female rats were ran-domly divided into 5 groups： control group, model group, low dose group, middle dose group and high dose group with 15 rats in each group. After 15 days of gavage, male and female rats were mated in the same cage. On the ninth day of gestation, all the rats were sacrificed. Body of weight and total organ coefficient （ pregnant uterine total coefficient ） were examined. Levels of serum rat β chorionic gonadotropin and pro-gesterone were determined by ELISA Kits. The mRNA levels of Bax, Bcl-2 and caspase-3 in embryos were determined by real time-PCR, and the expressions of Bax, Bcl-2 and caspase-3 were examined by Western blot. Results ①Compared with control group, the weight of pregnant rats, organ coefficient （ pregnant u-terine total coefficient） , levels of serum rat β chorionic gonadotropin and progesterone in BaP group were de- creased significantly, mRNA and protein expression of Bcl-2 decreased dramatically, while mRNA and protein expression of Bax and caspase-3 increased significant- ly. ②After treatment with SCE the weight of pregnant rats, organ coefficient （pregnant uterine total coeffi- cient）, levels of serum rat 13 chorionic gonadotropin and progesterone were increased, mRNA and protein expression of Bcl-2 increased significantly compared with BaP group, while mRNA and protein expression of Bax and caspase-3 were decreased. Conclusion BaP exposure before pregnancy can generate embryo toxicity in rats. SCE can inhibit the reproductive damage in- duced by BaP, and this positive effect may be related to the cell apoptosis mechanism.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2015年第3期381-387,共7页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No 81273977)
天津市自然科学基金资助项目(No 12JCYBJC16200)
