摘要
目的观察中药复方联合三苯氧胺(TAM)对MCF-7乳腺癌细胞周期及细胞周期素Dl(Cyclin D1)、Bcl-2、Bax表达的影响。方法将造模成功的裸鼠随机分为模型组、三苯氧胺(TAM)组、乳结合组(乳岩宁方+TAM)和益结合组(益气养阴方+TAM)。连续给药21 d后脱颈处死裸鼠,完整剥离出瘤体,切开标本取材。采用流式细胞仪(FCM)检测乳腺癌细胞周期的分布,Western blotting法检测瘤组织中Bax、Bcl-2、Cyclin D1蛋白表达。结果各用药组G0/G1期细胞的比例增多,S期比例明显减少,与模型组比较差异有统计学意义(P<0.01);但是乳结合组与益结合组分别对比G0/G1期、S期的细胞比例均无统计学差异(P>0.05)。各用药组均可下调Bcl-2及Cyclin D1蛋白的表达,上调Bax蛋白的表达,降低Bcl-2/Bax的比值,与模型组相比差异有统计学意义(P<0.01);两个结合组与TAM组比较,差异有统计学意义(P<0.01);乳结合组与益结合组之间比较,差异无统计学意义(P>0.05)。结论中药复方联合TAM通过阻滞细胞周期进程、下调细胞周期相关蛋白Cyclin D1、抑制Bcl-2的表达、促进Bax的表达来抑制MCF-7乳腺癌细胞增殖,同时中药复方对TAM有协同作用。
Objective To observe Chinese herbal compound recipe and Tamoxifen on cell cycle and the expressions of Cyclin D1,Bcl-2 and Bax protein of MCF-7 in breast cancer. Methods Mice were randomly divided into the model group,Tamoxifen（ TAM） group,Ru combined group（ Ru yanning Recipe ＋ TAM）and Yi combined group（ Yi qixiaoji Recipe ＋ TAM） after modeling successfully. All the mice were sacrificed through pull off neck after given drug for 21 days,tumor were taken completely,then cut for different specimens. The distribution of cell cycle was detected by Flow cytometry; the expression of Bcl-2,Bax and Cyclin D1 proteins was detected by Western blot method. Results In each treatment group,the proportion of cells in G0/ G1 phase was increased,S phase was sharply reduced compared with the model group（ P 〈 0. 01）,but there was no statistical difference between Ru combined group and Yi combined group about cell ratio in the G0/ G1 phase and S phase（ P 〉 0. 05）; The expression of Bcl-2 and Cyclin D1 protein was down-regulated,Bax was up-regulated in the three treatment groups,the ratio of Bcl-2 / Bax was significantly decreased,the difference was significant compared with the model group（ P 〈 0. 01）,there was also significant difference between two combined groups and TAM group（ P 〈 0. 01）,but there was no significant difference between Ru combined group and Yi combined group（ P 〉 0. 05）. Conclusion Chinese herbal compound formula has function of cell proliferation inhibition,the possible mechanism is related to blockcell cycle at G0/ G1 phase,down-regulate expression of Cyclin D1,Bcl-2 protein and up-regulate the expression of Bax protein. There is synergy between Chinese herbal compound formula and TAM.
出处
《上海中医药杂志》
2015年第4期83-86,共4页
Shanghai Journal of Traditional Chinese Medicine
基金
辽宁省卫生厅高峰工程项目(2010055)
