摘要
采用水提醇沉法、单因素正交试验研究黄花菜叶多糖(polysaccharides from Daylily leaves,PDL)的提取工艺,通过考察PDL对3种自由基的清除效果来研究其抗氧化活性。结果显示,PDL最优提取条件为提取温度90℃,提取时间3 h,液固比30:1(m L/g),提取3次。在此条件下,PDL提取率为9.13%,多糖含量为6.05%。PDL对O2-·和·OH的清除率分别为55.31%和19.45%,对脂质过氧化物(lipid peroxide,LPO)的抑制率为42.17%。综上表明,PDL对3种自由基的清除效果不显著,其抗氧化活性不强。
The extraction of polysaccharides from Daylily leaves（PDL） by water-extracting and alcohol-precipitating was optimized using one-factor-at-a-time and orthogonal array method. Meanwhile, the antioxidant activity of PDL by its scavenging effect on three free radicals was investigated. The results showed that the optimal conditions for PDL extraction was determined as three extraction cycles with distilled water at a solvent/solid ratio of 30：1（m L/g） at 95℃ for 3 h. Under these conditions, the extraction yield of PDL was 9.13%, the content of PDL was 6.05%. The scavenging rate of PDL for O2-·, ·OH and lipid peroxide（LPO） was 55.31%,19.45% and 42.17%, respectively. All main findings showed that PDL had little scavenging effect on three free radicals and weak antioxidant activity.
出处
《襄阳职业技术学院学报》
2015年第2期26-30,共5页
Journal of Xiangyang Polytechnic
关键词
黄花菜
多糖
提取工艺
抗氧化活性
Daylily
polysaccharides
extraction process
antioxidant activity
