摘要
目的探讨mi R-183及Akt1蛋白在非小细胞肺癌(NSCLC)组织中的表达,以及抑制或过表达mi R-183对Akt1蛋白表达的影响。方法实时定量PCR方法检测NSCLC组织mi R-183m RNA及Akt1m RNA的表达水平,Western blot方法检测NSCLC组织Akt1蛋白表达;将mi R-183 inhibitors及mi R-183 mimics转染至A549细胞,实时定量PCR方法及Western blot方法分别检测转染后Akt1 m RNA及蛋白的表达变化。结果 mi R-183m RNA在非小细胞肺癌组织中的表达量较癌旁组织明显降低(<0.01),Akt1 m RNA及蛋白在癌组织中较癌旁组织表达明显增高(<0.05);转染mi R-183 inhibitors的细胞内Akt1蛋白与m RNA水平与对照组相比显著升高(<0.05),转染mi R-183 mimics的细胞内Akt1蛋白与m RNA水平对照组相比显著降低(<0.05)。结论 mi R-183在NSCLC组织中低表达,并能够调节Akt1的表达。
Objective To investigate the expressions of mi R-183 m RNA and Akt1 m RNA and protein in non small cell lung cancer（NSCLC）tissue, and the effect of inhibitors or mimics of mi R-183 on the expression of Akt1. Methods The expressions of mi R-183 m RNA and Akt1 m RNA and protein were detected by real-time quantitative PCR and Western blot respectively after the mi R-183 inhibitors or mi R-183 mimics was transfected into A549 cells. Results The level of mi R-183 m RNA was significantly decreased in non small cell lung cancer tissues than in normal tissues（P〈 0.01）. The expression level of Akt1 protein and m RNA was significantly higher in cancer tissues than in normal tissues（ P〈0.05）, but increased significantly after mi R-183 inhibitors was transfected into A549 cells（P〈 0.05）, however decreased significantly after mi R-183 mimics was transfected into A549 cells（ P〈0.05）. Conclusion mi R-183 m RNA was downexpressed in NSCLC tissues, but can regulate the expression of Akt1.
出处
《解剖科学进展》
CAS
2015年第2期174-177,共4页
Progress of Anatomical Sciences
基金
辽宁省科技攻关项目(No.2013225021)
