摘要
为了更有效地保护、管理和繁育缅甸蟒(Python bivittatus)濒危物种,有必要利用分子技术对地方物种多样性和遗传分化进行研究。试验采用TA克隆双向测序方法对来自海南的37条缅甸蟒和来自越南的28条缅甸蟒部分控制区II序列进行了测定。结果表明,两个地理群体共分为25个单倍型,单倍型多样度(Hd)为0.877±0.025,核苷酸多样性(π)为0.004 76±0.000 41,平均核苷酸差异数(k)为2.676,显示缅甸蟒整体遗传多样性不高;通过比较两群体遗传多样性,发现海南缅甸蟒的单倍型多样度要高于越南缅甸蟒,但核苷酸多样性和平均核苷酸差异数低于越南缅甸蟒;基于控制区构建ML树和Network网络分析显示,两群体均各自形成了明显的两大分支;通过计算得到两群体的固定指数(Fst)为0.201(P<0.001),显示海南缅甸蟒与越南缅甸蟒存在极显著差异。
It was necessary to study genetic diversity and differentiation of local species with molecular techniques for effectively protecting, managing and breeding Burmese python（Python bivittatus）. TA bidirectional sequencing was used to obtain part of mt DNA control region II of P.bivittatus from 37 Hainan individuals and 28 Vietnam individuals. A total of 25 haplotypes were obtained. Haplotype diversity（Hd）, nucleotide diversity π and the average number of nucleotide difference k were0.877±0.025, 0.004 76±0.000 41 and 2.676, respectively, indicating that the genetic diversity of P.bivittatus was not very high.By comparing the genetic diversity of the two populations, the diversity of haplotype in P.bivittatus from Hainan was higher than that from Vietnam. The nucleotide diversity π and the average number of nucleotide difference k of the former population was lower. ML trees and network analysis based on mt DNA control region showed that the two populations were divided into 2 clades. The fixation index（Fst） reached 0.201（P0.001）, indicating that there were significant differences between the two populations.
出处
《湖北农业科学》
2015年第2期398-401,408,共5页
Hubei Agricultural Sciences
基金
海南省重点科技计划项目(ZDXM20120007)
