摘要
目的比较白木香结香前后内生真菌群落结构和变化,探讨内生真菌对白木香结香的影响。方法分别提取白木香结香前后总DNA,采用真核生物r DNA ITS区通用引物ITS1F-gc/ITS2进行扩增,变性梯度凝胶电泳(Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis,PCR-DGGE)分离后,切割回收条带进行克隆、测序和比对。结果 PCR-DGGE图谱聚类表明,未结香组与结香组分别聚在一起;与未结香组比较,结香组基因型丰富度、香农多样性指数显著提高(P<0.01);回收条带测序比对结果多为真菌,极个别序列无比对结果。结论白木香结香前后内生真菌群落存在差异,内生真菌可能参与白木香的结香过程。
Objective To investigate and compare the community structure of endophytic fungi in Aquilaria sinensis before and after agarwood formation,and to study the effect of endophytic fungi on agarwood formation.Methods Total DNA was extracted from Aquilaria sinensis before and after agarwood formation.The universal primer ITS1F-gc/ITS2 in fundi r DNA ITS section was amplified,and then the amplified product was isolated by polymerase chain reaction-denaturing gradient gel electrophoresis（PCR-DGGE）.The recovery band was cloned,sequenced and compared.Results The clustering patterns of PCR-DGGE revealed that the profiles of agarwood group and normal white wood group were classified into the corresponding groups.Agarwood group had richer Genotypes and higher Shannon＇s diversity index than normal white wood group（P〈0.01）.In addition,the results of comparison in recovery bands showed that that most of the bands were confirmed as fungi,fewer sequences had no comparison results.Conclusion Endophytic fungi community varies Aquilaria sinensis before and after agarwood formation,indicating that the endophytic fungi may be involved in the process of agilawood formation.
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2015年第2期221-225,共5页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
广州中医药大学优秀青年学者科研基金(KAB108133K11)
广东省教育厅育苗工程基金(2013LYM_0011)
关键词
白木香
内生真菌
沉香
变性梯度凝胶电泳
Aquilaria sinensis（Lour.）Gilg
Endophytic fungi
Agarwood
Polymerase chain reaetion-denaturing gradient gel electrophoresis
