摘要
[目的]观测眼针干预脑缺血再灌注模型大鼠抗氧化机制。[方法]使用随机平行对照方法,60只大鼠适应性饲养1周,采用完全随机法分为4组:空白对照组10只,假手术组10只,脑缺血再灌注模型组20只,眼针治疗组20只。脑缺血再灌注模型组,眼针治疗组参照线栓法制备大鼠脑缺血再灌注模型方法复制脑缺血再灌注大鼠模型。模型复制成功,栓塞2h后,拔除栓塞线,进行再灌注,再灌注时间为72h;空白对照组,常规饲养,不施加任何干预;假手术组手术方式同脑缺血再灌注模型组,但不做栓塞,仅暴露出右侧颈总动脉(CCA)、颈外动脉(ECA)、颈内动脉(ICA),然后逐层缝合即可。空白对照组、假手术组及脑缺血再灌注模型组,模型复制后,正常饲养,不做任何处理。眼针治疗组,模型复制成功后,再灌注即刻开始进行眼针针刺治疗,取13mm毫针,眼针组于大鼠眶周2mm处针刺,定位参照人体取穴方法,取肝区、上焦区、下焦区、肾区。针刺手法:从眼眶边缘2mm部位平刺,左眼按照顺时针方向(右侧按逆时针方向),从该穴区起始定位线向终止定位线进针,进行平刺操作,刺入真皮,达到皮下组织,进针3mm,到终止定位线为止。留针20min,留针10min时用刮柄进行刮针1次,刮针5下,2次/d,连续3d。观测MDA、SOD、GSH-PX指标、死亡率。[结果]死亡率空白对照组、假手术组为0,脑缺血再灌注模型38.90%,眼针治疗组23.50%。脑缺血再灌注模型组神经功能缺损评分高于空白对照组(P<0.01);与脑缺血再灌注模型组相比,眼针治疗组神经功能缺损评分明显降低(P<0.01)。脑缺血再灌注模型组、眼针治疗组大鼠血清MDA含量高于空白对照组和假手术组(P<0.01),眼针治疗组大鼠血清中MDA含量低于脑缺血再灌注模型组(P<0.01);脑缺血再灌注模型组、眼针治疗组大鼠血清SOD和GSH-PX含量低于空白对照组和假手术组(P<0.01),眼针治疗组大鼠血清SOD和GSH-PX含量高于脑缺血再灌注模型组(P<0.01)。[结论]眼针能通过调节抗氧化产物含量表达途径,降低脑缺血再灌注后损伤。
[Objective] Observation of eye acupuncture intervention on cerebral ischemia reperfusion model rats antioxidation mechanism. [Method] Using random parallel control Method, 60 rats 1 week adaptive feeding. Divided into 4 groups by completely randomized： blank control group 10, 10 rats in the sham operation group, cerebral ischemia reperfusion 20 rats in model group, acupuncture group 20.Ischemia-reperfusion model group, acupuncture group reference suture preparation of rat cerebral ischemia reperfusion model Method to study replication cerebral ischemia reperfusion rat model. Model successfully reproduced, embolism after 2h, removal of embolism line, reperfusion, the reperfusion time was 72 h. The blank control group, conventional breeding, does not impose any intervention. Mode of operation in sham operation group with cerebral ischemia reperfusion model group, but not embolism, only exposed the right common carotid artery（CCA）, external carotid artery（ECA）, internal carotid artery（ICA）, and then sutured to. The blank control group, sham operation group and cerebral ischemia reperfusion model group, model after the copy, the normal feeding, without any treatment. Eye acupuncture treatment group, model successfully reproduced, reperfusion immediately began to eye acupuncture therapy, 13 mm needle, eye needle group of rats in the periorbital 2mm acupuncture, acupoint selection Method according to human orientation, to take the liver area, the lower the focal region, the kidney area. Acupuncture manipulation： from the orbital edge part 2mm flat spines, left in a clockwise direction（right in an anti clockwise direction）, the needle from the starting location of the acupoint thread to terminate the positioning line for flat, sting operation, piercing the leather, reach subcutaneous tissue, the needle 3mm, to terminate the positioning line so far. For 20 min, 10 min when using the handle of the needle retaining scraping scraping the needle 1, scraping the needle 5, 2 times /d, continuous 3D. Observation of MDA, SOD, GSH-PX index, mortality. [Results] The mortality of blank control group, sham operation group was 0, 38.90% models of cerebral ischemia reperfusion, treatment group 23.50% eye needle. Cerebral ischemia reperfusion model group of nerve function deficit score higher than the control group（P〈0.01）; and the model of cerebral ischemia reperfusion group than in acupuncture group, the score of neural function defect decreased significantly（P〈0.01）. Cerebral ischemia reperfusion model group, the serum MDA content of eye acupuncture treatment group rats were higher than those in the blank control group and sham operation group（P〈0.01）, eye of the needle treatment group rats serum MDA content was lower than that of cerebral ischemia reperfusion model group（P〈0.01）; cerebral ischemia reperfusion model group and eye acupuncture serum SOD and GSH-PX content in rats was lower than the blank control group and sham operation group（P〈0.01）, eye treatment serum SOD and the content of GSH-PX group rats was higher than that of cerebral ischemia reperfusion model group（P〈0.01）. [Conclusion] Eye of the needle through the regulation of antioxidant product content expression way, reduce after cerebral ischemia reperfusion injury.
出处
《实用中医内科杂志》
2015年第2期124-126,156,共4页
Journal of Practical Traditional Chinese Internal Medicine
