摘要
目的研究不同施硒水平下接种AM真菌对丹参幼苗品质的影响。方法利用盆栽试验得到不同生长条件下丹参幼苗,通过测定多糖、硒、丹参酮ⅡA含量评价丹参品质。结果同一施硒水平下,接种AM真菌显著提高丹参幼苗多糖、微量元素硒的质量分数,表明AM真菌能够促进丹参对营养物质的积累,增强其对微量元素的吸收。且接种株丹参酮ⅡA质量分数均高于未接种株,当硒质量浓度为40μg/m L时接种株丹参酮ⅡA质量分数最高,为0.272%;不同施硒水平下,随硒质量浓度的增加,丹参多糖与丹参酮ⅡA含量均先增大后减小,但接种株含量高于未接种株,在Se1（20μg/m L）~Se2（40μg/m L）时达到最高,说明高硒反而抑制药用成分的富集。由此看出适量质量浓度的硒与AM真菌具有协同作用,能够更有效地促进丹参营养和药用成分的积累。丹参中硒质量分数与施硒质量浓度呈显著正相关,但无明显线性关系,且硒的分布为茎叶〉根部。结论硒质量浓度为20~40μg/m L时AM真菌对丹参的接种效果最佳。
Objective To explore the effects of AM fungi on the quality of Salvia miltiorrhiza under different selenium levels. Methods Salvia miltiorrhiza seedlings under different conditions were obtained by pot experiment, and the content of polysaccharide, selenium and tanshinone IIA were determinated to evaluate the quality of plants. Results Inoculation of AM fungi significantly increased polysaccharide and selenium content of Salvia miltiorrhiza under the same selenium level. It can be seen AM fungi can promote the accumulation of nutrients, improve the uptake of nutrient elements. The contents of tanshinone IIA in inoculated plants were higher than non inoculated plants. While the selenium concentration was 40 μg/mL, the content of tanshinone IIA in inoculated plants was highest, that was 0.272%. On the different selenium levels, the content of polysaccharide and tanshinone IIA increased firstly and then decreased with increasing selenium concentration, but the contents of inoculated plants were higher than non inoculated plants, they reached the maximum at Sel（20 μg/mL）-Se2（40 ktg/mL）, showed that high selenium inhibited the accumulation of medicinal components. From that proper concentration of selenium was cooperated with AM fungi on improving the accumulating of polysaccharide and medicinal components of Salvia miltiorrhiza. Selenium content in Salvia miltiorrhiza Bge was positively correlated with selenium concentrations, but no obvious linear relationship, the distribution was more in the leaf than root. Conclusion Inoculation of AM fungi on the Salvia miltiorrhiza had the best effects when the selenium concentration was 20-40 μg/mL.
出处
《医学研究与教育》
CAS
2015年第2期1-5,共5页
Medical Research and Education
基金
河北省自然科学基金资助项目(C2010000273)
关键词
AM真菌
丹参
多糖
硒
丹参酮ⅡA
AM fungi
Salvia miltiorrhiza Bge
polysaccharide
Selenium
tanshinone ⅡA
