摘要
小麦WRKY转录因子在抗旱抗逆等方面有重要作用。为小麦抗旱抗逆分子模块组装育种提供参考,本研究在已有小麦TaWRKY2基因cDNA序列（GenBank登录号：EU665425）的基础上,从小麦品种晋麦79号中克隆获得TaWRKY2基因全长序列,并对TaWRKY2的基因结构、基因组信息进行研究,同时检测其在部分小麦亲缘种、黄淮麦区水旱地代表品种、本课题组选育的高代品系及部分水旱地杂交组合F1代中的分布。结果表明,克隆所得到的TaWRKY2基因全长DNA序列包含4个外显子和3个内含子,其中,3个内含子长度变化范围为111~172bp;外显子和内含子的交接处序列符合GT-AG原则;与乌拉尔图小麦（Triticum urartu,AA）和粗山羊草（Aegilops tauschii,DD）中的片段序列同源性分别为93%和99%,与中国春小麦（AABBDD）1AS、1BS和1DS染色体的片段序列同源性分别为93%、88%和99%。除野生一粒小麦外,在小麦进化祖先种、黄淮海水旱地推广的代表品种、课题组选育的高代品系及F1代共78份材料中,都能够检测出该基因的普遍存在,说明该基因可应用于小麦抗旱抗逆分子设计育种中。
WRKY transcription factors play important roles in plant responses to drought and other stresses. In order to provide the theoretical basis for its application in module assembly of wheat mo- lecular breeding, TaWRKY2 were cloned from Jinmai 79 according to its cDNA sequence （GenBank accession number EU665425）,the gene structure and genomic comparison of TaWRKY2 were ana- lyzed,and detected in 79 wheat germplasm including the ancient species,modern varieties （lines） and hybrid F1 generations. The results showed that Ta WRKY2 gene cloned from Jinmai 79 contained 4 ex- ons and 3 introns,and the splicing principles of its exons and intron were consistent with GT-AG,the size of 3 introns ranged from 111 bp to 172 bp. TaWRKY2 sequences were with high similarity to the segment in Triticum urartu （AA）,Aegilops tauschii （DD）, with similarity of 93% and 99% respectively,and the similarity between its segment in 1AS, 1BS and 1DS chromosome segments of Chinese Spring （AABBDD） was 93%, 88% and 99%, respectively. Except Triticum boeoticum, TaWRKY2 was detected by PCR in 78 wheat materials including the ancient species,modern varieties （lines） and hybrid F1 generations. That means Ta WRKY2 gene is common in wheat and can be used in molecular design breeding for drought and other stress resistance.
出处
《麦类作物学报》
CAS
CSCD
北大核心
2015年第5期585-590,共6页
Journal of Triticeae Crops
基金
中国科学院战略性先导科技专项(A类)分子模块设计育种创新体系(XDA0803010703)
中国科学院生物局知识创新重要方向性项目小麦新品种培育及资源高效利用(KSCX2-EW-N-02)
