摘要
背景:马钱子的主要有效成分为总生物碱,主要功效是通络止痛、消肿散结、兴奋健胃、凉血等,对神经系统、心血管系统等也有一定的药理作用。目的:观察马钱子对实验性家兔骨折愈合过程的影响。方法:制备家兔桡骨骨折模型,随机分为给药组和生理盐水对照组,每组10只。室温条件下,对给药组家兔灌胃马钱子粉混悬液;对照组家兔灌胃生理盐水,1次/d。给药后7,14,28 d各作X射线影像学检查,比较对照组与给药组家兔骨折愈合情况。给药后14,28 d时耳缘静脉取血,与给药前作血清学比较,检测分析家兔骨折模型血清碱性磷酸酶,微量元素钙(Ca)、镁(Mg)、铜(Cu)、铁(Fe)、锌(Zn)质量浓度变化。结果与结论:家兔骨X射线影像学结果显示给药组家兔骨折愈合速度比对照组快。血清学检查结果表明,给药后14和28 d给药组家兔血清中碱性磷酸酶浓度明显高于给药前和对照组(P<0.05)。微量元素中,对照组家兔给予生理盐水后28 d时血清Fe质量浓度高于给生理盐水前(P<0.05);给药组家兔给药后14 d和28 d血清中Ca、Zn水平均高于给药前和对照组;给药后14 d Mg,Fe质量浓度明显高于给药前和同时间点对照组(P<0.01,P<0.05);给药后28 d Fe质量浓度明显高于给药前(P<0.01)。说明马钱子可以增加骨折家兔碱性磷酸酶和微量元素水平,对家兔骨折愈合有促进作用。
BACKGROUND：The main active ingredient of nux vomica is total alkaloids that can activate meridian to stop pain, reduce sweling, excite stomach and cool blood, and also have some pharmacological effects on the nervous system and cardiovascular system. OBJECTIVE：To investigate the effects of nux vomica on the fracture healing process of experimental rabbits. METHODS： Rabbit models of radius fracture were established and then randomized into drug administration group and control group, with 10 animals in each group. At room temperature, the nux vomica powder mixed suspension was administrated into the rabbits in the drug administration group, while the normal saline was administrated into the rabbits in the control group, once a day. X-ray examination was performed at 7, 14, 28 days after administration to compare the fracture healing between the two groups. Blood samples from ear vein were taken at 14 and 28 days after drug administration for serological comparison to analyze the concentration changes of serum alkaline phosphatase and trace elements including Ca, Mg, Cu, Fe, Zn. RESULTS AND CONCLUSION：Rabbit bone X-ray examinations results showed that compared with the control group, the fracture healing of the drug administration group was faster. The concentration of serum alkaline
phosphatase of rabbits in the drug administration group at 14 days and 28 days after drug administration were higher than that before drug administration and in the control group at the same time respectively （P 〈 0.05）. The serum Fe concentration of rabbits in the control group at 28 days after normal saline administration was higher than that before normal saline administration （P 〈 0.05）. The serum Ca and Zn levels of rabbits in the drug administration group at 14 days and 28 days after drug administration were higher than those before drug administration and the control group at the same time, respectively （P 〈 0.05）. The Mg and Fe levels of rabbits in the drug administration group at 14 days after drug administration were significantly higher than those before drug administration and those in the control group at the same time, respectively （P 〈 0.01,P 〈 0.05）. The Fe level of rabbits in the drug administration group at 28 days after drug administration was significantly higher than that before drug administration （P〈 0.01）. Nux vomica can increase the alkaline phosphatase and trace elements levels, and play a promoting role in the process of healing.
出处
《中国组织工程研究》
CAS
北大核心
2015年第11期1647-1651,共5页
Chinese Journal of Tissue Engineering Research
基金
武汉市科技局国际合作研究项目(200870834408)~~
