摘要
目的:比较超滤法与醇沉法对黄芪与红芪保护脑缺血大鼠作用的影响。方法:将大鼠分为黄芪与红芪水煎液、醇沉物、超滤物及正常、模型等组,在给药的基础上注射地塞米松来复制血瘀证模型,实验结束时结扎颈总动脉来复制脑缺血模型,通过观测脑组织代谢水平及抗氧化系统的活性,研究超滤法与醇沉法对黄芪与红芪抗脑缺血作用的影响。结果:6 g(原生药)/kg黄芪、红芪的水提取物及其水提醇沉物与超滤物可明显提高缺血脑组织中ATP的含量与SOD、CAT的活性,降低MDA的含量,6 g(原生药)/kg黄芪、红芪的水提取物及其超滤物可降低脑组织中乳酸以及脑组织含水量;与黄芪、红芪的水提物相比,在脑组织MDA与含水量、SOD、CAT的活性等方面,黄芪、红芪的水提醇沉物的作用明显减弱。结论:黄芪、红芪水提液及其醇沉液与超滤液具有明显的抗脑缺血作用且相同提取方法所得黄芪与红芪的提取物之间的作用差异不明显;醇沉法对黄芪、红芪的抗脑缺血作用有明显削弱的影响。
Objective： To investigate the influences of ultrafiltration and alcohol sedimentation on protective effects of Radix Astragali and Radix Hedyseri against rat＇s cerebral ischemia. Methods： Using dexamethasone（im. ） and ligating common carotid artery, the rat stasis model combined transient cerebral ischemia was established to evaluate the effects of the ultrafiltration and alcohol sedimentation through detecting an- tioxidant system and other indexes in brain tissue. Results： The results showed that the 6 g/kg water extract（crude drug）, ultrafiltration and alcohol sedimentation of Radix Astragali and Radix Hedyseri could upgrade adenosine-triphosphate（ATP）, superoxide dismutase（SOD） and catalase（CAT）, and degrade malondialdehyde（MDA） and water content of brain tissue in rat stasis model combined transient cerebral ischemi- a, the water extract and ultrafiltration of them could degrade lactic acid（LD） of brain tissue, and the effects of alcohol sedimentation of Radix Astragali and Radix Hedyseri become weaker than water extract of them. Conclusion： The water extract, ultrafiltration and alcohol sedimenta- tion of Radix Astragali and Radix Hedyseri have some protective effects on cerebral ischemia in rats, the effective differences of the extract through the same extraction method are not remarkable, and alcohol precipitation method has obvious influences effect on Radix Astragali and Radix Hedyseri.
出处
《中国应用生理学杂志》
CAS
CSCD
2015年第2期132-135,共4页
Chinese Journal of Applied Physiology
关键词
黄芪
红芪
醇沉法
超滤法
脑缺血
大鼠
Radix Astragali（RA）
Radix Hedyseri（RH）
alcohol precipitation method
uhrafiltration
cerebral ischemia
rat
