摘要
目的 鉴定引起2010年湖北省急性出血性结膜炎（acute hemorrhagic conjunctivitis,AHC）疫情的病原体,并对其进行基因进化分析.方法 采集20份门诊AHC患者眼结膜拭子,对其进行病毒分离,随后通过PCR方法分别检测阳性分离物中肠道病毒70型（human enterovirus D-70,EV-D70）和柯萨奇A24变异株（Coxsackievirus A24 variant,CV-A24v）.对CV-A24v分离株进行VP1区和3C区全序列测定,分析其与全球流行CV-A24v的基因进化关系.结果 20份标本中有11份病毒分离阳性,PCR检测表明这11份病毒分离物为CV-A24v.基于3C区和VP1区构建的基因进化树表明湖北CV-A24v属于GenotypeⅣ基因型的Cluster 3（GⅣ-C3）,而且在GⅣ-C3内湖北CV-A24v分属于A和B两个传播链.结论 本研究表明2010年至少有两个CV-A24v传播链在湖北同时流行.
Objective To identify the etiology of an outbreak of cute hemorrhagic conjunctivitis （AHC） occurred in Hubei in 2010 and analyze the molecular evolution of these viruses.Methods Conjunctival swabs from 20 eyes were collected from AHC patients,performed virus isolation was performed and then these specimens were tested for human enterovirus 70 （EV-D70） and Coxsackievirus A24 variant （CV-A24v） using polymerase chain reaction （PCR） technique.All CV-A24v isolates were subjected to 3C and VP1 coding region sequencing and phylogentic tree were constructed for Hubei CV-A24v and other CV-A24v worldwide based on 3C and VP1 region,respectively.Results The results showed that 11 out of 20 specimens were positive for virus isolation and identified as CV-A24v.The phylogenetic tree based on 3C region showed Hubei CV-A24v belong to Cluster 3 in Genotype IV （GIV-C3）with strains isolated throughout the world after 2010 and further divided into A and B lineages.Conclusions This study suggested that at least two transmission lineages circulated in HuBei province in 2010 causing the AHC outbreak nationwide.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2015年第2期109-111,共3页
Chinese Journal of Experimental and Clinical Virology
基金
国家自然科学基金（项目批准号81373049）
