摘要
目的:探讨A型肉毒毒素(botulinum toxin A,BTXA)对增生性瘢痕组织成纤维细胞中TGF-β1和Ⅰ型胶原蛋白表达的影响。方法:组织块法培养增生性瘢痕组织及正常皮肤组织的成纤维细胞,用不同浓度的BTXA作用于体外培养的人增生性瘢痕成纤维细胞,检测MTT值;将浓度为0.2U/ml、0.4U/ml、0.8U/ml BTXA作用于增生性瘢痕成纤维细胞48h,利用RT-PCR检测BTXA对TGF-β1和Ⅰ型胶原蛋白m RNA的表达量。结果:BTXA在体外能明显抑制增生性瘢痕成纤维细胞的增殖,随着药物浓度的增加及时间的延长,其抑制作用明显增强,能够减少TGF-β1和Ⅰ型胶原蛋白m RNA的表达,且随着药物浓度的增加,TGF-β1和Ⅰ型胶原蛋白m RNA的表达量呈下降趋势。结论:BTXA通过抑制瘢痕组织中成纤维细胞的增殖,减少TGF-β1和Ⅰ型胶原蛋白m RNA的表达,减少细胞外基质的异常沉积来影响增生性瘢痕的形成。
Objective To investigate the effect of botulinum toxin A （BTXA）on the expression of TGF-β1 and collagenⅠin hypertrophic scar fibroblast. Methods After treated with different concertrations of BTXA,the inhibition rate of hypertrophic scar fibroblasts were detected by MTT assay,0.2U/ml,0.4 U/ml and 0.8U/ml of BTXA was added in cultured hypertrophic scar fibroblast for 48h,the expression of TGF-β1 and collagenⅠin hypertrophic scar fibroblasts was respectively detected with RT-PCR. Results BTXA can significantly inhibit the proliferation of hypertrophic scar fibroblast,and the expression of TGF-β1and collagenⅠcould be inhibited significantly,the levels of the proliferation of hypertrophic scar fibroblast and the expression of TGF-β1 and collagenⅠdecreased along with the increase in BTXA concertration. Conclusion BTXA inhibits hypertrophic scar formation through inhibiting the proliferation of hypertrophic scar fibroblast and down-regulating the expression of TGF-β1 and collagenⅠ.
出处
《中国美容医学》
CAS
2015年第7期40-43,共4页
Chinese Journal of Aesthetic Medicine
