摘要
目的：建立高效的红花（Carthmus tinctorius L.）离体组织培养体系,为红花的基因操作搭建平台。方法：以萌发6~8d的红花无菌苗子叶为外植体,选用MS基础培养基,应用萘乙酸（1-naphthylacetic acid,NAA）＋6-苄氨基嘌呤（6-benzyl amino purine,6-BA）＋激动素（kinetin,KT）为组织培养的植物生长调节剂,优化红花的组织培养体系。结果：红花组织培养的最佳植物生长调节剂配方为：NAA 2.0mg/L＋6-BA 4.0mg/L＋KT 20.0mg/L,添加活性炭（4g/L）或硝酸银（5mg/L）,光照时间为16h/d,光照强度9000lx,培养温度为（25±2）℃,平均愈伤组织发生率高达86.4%,而且再生芽生长稳定,重现性高。结论：本研究优化建立了红花的不定芽再生体系,为红花基因操作平台的建立奠定了基础。
Objective：To establish the regeneration system of safflower（Carthamus tinctorius L.）,so as to create a platform of gene manipulation of safflower.Methods：Through tissue culture experiments,cotyledons germinated 6-8 days of safflower were selected as explants and cultured with Murashige and Skoog（MS）as the basal medium at（25±2）℃temperature with 16h/d illumination and 9000 lx light intensity.Results：The optimum callus medium of safflower was MS basal medium supplemented with 1-naphthylacetic acid（NAA）2.0 mg/L＋6-benzyl amino purine（6-BA）4.0mg/L＋kinetin（KT）20.0mg/L and addition of acticarbon（4g/L）or silver nitrate（5mg/L）,which made the callus mean rate as high as 86.4%and with higher regeneration bud grow stability and reproducibility.Conclusion：The research optimized adventitious shoot regeneration system and established the foundation for safflower gene manipulation platform.
出处
《药学服务与研究》
CAS
2015年第2期91-94,共4页
Pharmaceutical Care and Research
基金
国家自然科学基金(81173484
81473300)
上海市自然科学基金(13ZR1448200)
关键词
红花
组织培养
萘乙酸
6-苄氨基嘌呤
激动素
Carthamus tinctorius L.
tissue culture
1-naphthylacetic acid
6 benzyl amino purine
kinetin
