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甘蓝Ogura胞质雄性不育系发生同源异型转化的分子机制研究 被引量：3

Study on Molecular Mechanism of Homeosis in Cabbage Ogura CMS
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摘要 为了揭示甘蓝Ogura胞质雄性不育系发生线粒体同源异型转化的分子机制,利用RT-PCR和转基因的方法分别进行了研究。RT-PCR试验结果表明,甘蓝B基因AP3在雌蕊(心皮)发育初期异位高表达,C基因AG3在发生心皮化的雄蕊部位异位高表达,这可能导致了甘蓝Ogura胞质不育系线粒体同源转化的发生。而拟南芥转基因试验表明,线粒体定位表达载体p3301-ATPORF138能导致拟南芥雄性不育的发生,这说明orf138基因是发生线粒体同源异型转化的甘蓝Ogura胞质雄性不育系不育的决定因素。 RT-PCR and transgenic method were used to elucidate the molecular mechanism of mitochondria homeosis in cabbage Ogura CMS. The RT-PCR research indicated that the high ectopic expression of homeobox gene B（AP3） was discovered in green pistil（carpel）,and which of C gene（AG3） was discovered in carpelloid stamen, which probably caused mitochondria homeosis in cabbage Ogura CMS. In addition,the test with transgenic Arabidopsis with mitochondria-directed expression vector p3301-ATP-ORF138 indicated that ORF138 could cause male sterile of Arabidopsis,which revealed that orf138 was a determinant factor of male sterility in cabbage Ogura CMS with mitochondria homeosis.
作者 张强 张晓伟 原玉香 姚秋菊 赵艳艳 蒋武生 魏小春 王志勇
机构地区 河南省农业科学院园艺研究所
出处 《河南农业科学》 CSCD 北大核心 2015年第5期111-116,共6页 Journal of Henan Agricultural Sciences
基金 国家自然科学青年基金项目(31101558) 河南省农业科学院优秀青年基金项目(2013YQ14) 农业部黄淮地区蔬菜科学观测试验站项目(河南)(1020520)
关键词 甘蓝 Ogura胞质雄性不育系 分子机制 同源异型转化 cabbage Ogura CMS molecular mechanism homeosis
分类号 S635 [农业科学—蔬菜学]
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参考文献31

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共引文献8

同被引文献52

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河南农业科学
2015年 第5期

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