摘要
以大蒜花序轴为试材,采用植物组织离体培养方法,研究了不同激素、蔗糖浓度及培养基的种类对花序轴分化及继代培养的影响。结果表明:以MS为基本培养基诱导大蒜花序轴分化的最佳培养条件为MS+6-BA 1.5mg/L+NAA 0.1mg/L,30g/L蔗糖,pH 5.8,分化率可达100%,增殖系数达46.3;最佳的继代培养基为1/2MS+6-BA 0.1mg/L+NAA 0.5mg/L,成活率可达100%,且长势最强。
Taking rachis garlic as test material,using plant tissue culture method,the effect of different concentrations of cytokinin,sucrose concentration and culture medium type on rachis of garlic were studied.The results showed that induction differentiation of rachis was the best when the medium was MS containing 6-BA 1.5mg/L,NAA 0.1mg/L and sucrose 30g/L at pH 5.8,the differentiation rate was 100%,the proliferation ratio was 46.3.The suitable subculture conditions were 1/2MS medium containing 6-BA 0.1mg/L,NAA 0.5mg/L,subsisr rate was 100%,and grew strongly.
出处
《北方园艺》
CAS
北大核心
2015年第10期108-110,共3页
Northern Horticulture
基金
黑龙江省财政厅基本业务专项基金资助项目(DJZ2011-04)
关键词
大蒜
花序轴
再生体系
Allium sativum L.
rachis
regeneration system
