摘要
目的:通过小鼠模型试验,探讨姜黄素对肥胖相关性肾小球病(ORG)的防治作用。方法:21只C57BL/6J雄性小鼠被随机分为对照组、ORG模型组及姜黄素干预组进行试验。在光镜下用计算机图像分析软件测量肾小球直径,用实时定量聚合酶链反应和Western印迹试验分别检测mRNA及蛋白表达。结果:与对照组相比,模型组小鼠体重、Lee指数、腹腔脂肪指数、尿蛋白定量、肾小球平均直径均显著增加;nephrin、podocin、podoplanin、podocalyxin的mRNA和蛋白表达均显著降低;肾组织Wnt1、Wnt2b、Wnt6及β-catenin的mRNA表达均显著上调;β-catenin蛋白的磷酸化水平显著下降。而姜黄素干预能够有效拮抗上述全部反应。结论:姜黄素能通过抑制Wnt/β-catenin信号通路激活而减轻肥胖及其肾损伤。
Objective：To study the effects of Curcumin intervention on mouse model of obesity related glomerulopathy （ORG）. Methods：Twenty one male C57BL/6J mice were randomly divided into control group（CG）,ORG model group（MG）and Curcumin intervention group. Glomerular diameters were measured by light microscopy. mRNA and protein expression was detected by real-time quantitative PCR and Western blotting,respectively. Results：Compared with the CG,body weight,Lee’s index,abdomi-nal fat index,urinary protein excretion,glomerular average diameter all were significantly increased in MG（P〈0. 05 or 0. 01）;Nephrin、podocin、podoplanin、podocalyxin mRNA and protein levels all were significantly decreased in MG（ P 〈0. 05 ）;Wnt1, Wnt2b,Wnt6 andβ-catenin mRNA expression of renal tissue was significantly up-regulated and phosphorylation level ofβ-cate-nin protein was significantly reduced in MG（P〈0. 05）. Curcumin intervention effectively antagonized the all above reactions（P〈0. 05）. Conclusion：Curcumin can relieve obesity and ORG by means of inhibition of Wnt/β-catenin signaling activation.
出处
《中国中西医结合肾病杂志》
2015年第4期291-294,I0001,共5页
Chinese Journal of Integrated Traditional and Western Nephrology
基金
中国博士后科学基金资助项目(No.2013M530667)
国家自然科学基金资助项目(No.81241115)
北京市中医药科技发展基金资助项目(No.JJ2012-20)
