摘要
采用分子印迹和SPR信号放大技术,构建一种以嵌有纳米金的分子印迹膜为识别元件,用于检测奶制品三聚氰胺的分子印迹膜-表面等离子体共振传感器（MIM-SPR）。首先利用表面热引发聚合的方法在SPR金片表面合成嵌有纳米金的分子印迹膜,再对MIM-SPR传感器的吸附响应性、选择性、再生性及精准度进行评价。该传感器的响应值与三聚氰胺在1×10^-2μg/m L浓度范围内线性关系良好,R^2=0.998。加标浓度在0.5~1.5μg/m L范围内的回收率为86.43%~107.11%,RSD小于6.0%（n=5）。结果表明该方法操作简单、选择性和再生性好、精准度高,可用于奶制品三聚氰胺残留量的测定。
Abstract.A molecularly imprinted-surface plasmon resonance（MIM-SPR） sensor for detecting melamine in dairy products was build by molecular imprinting and SPR signal enlarger technique,and the recognition element of this sensor was molecular imprinted membranes embedding with Au nanoparticles. First of all,the molecularly imprinted membranes embedding with Au nanoparticles were synthesized on the SPR gold chips through surface-initiated thermal polymerization ,and then the response ,selectivity, reproducibility and accuracy of the MNM-SPR sensors were evaluated respectively. The calibration curve of this detecting methods had showed good linearity（R^2=0.998）,when the concentration of melamine ranged from 1×10^-2μg/m L to 2μg/mL, and the recoveries were 86.43%-107.11%,when the spiked concentration ranged from 0.5μg/mL to 1.5μg/mL, and RSD less than 6.0%（n=5）. The results suggested that this method was easily operated,selective, reproducible,accurate and applicable for detecting melamine residue in dairy products.
出处
《食品工业科技》
CAS
CSCD
北大核心
2015年第12期70-73,共4页
Science and Technology of Food Industry
基金
渭南职业技术学院院级课题(WZYY201315)
关键词
表面等离子体共振
分子印迹膜
纳米金
信号放大
三聚氰胺
surface plasmon resonance
molecularly imprinted membranes
Au nanoparticles
signal enlarger melamine
