期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

羟基红花黄色素A通过抑制动脉内皮细胞中TNFR1/NF-κB信号通路而发挥抗炎作用 被引量：20

在线阅读 下载PDF
导出
摘要 目的研究羟基红花黄色素A(HSYA)对小鼠动脉血管内皮细胞肿瘤坏死因子α受体1/核因子κB(TNFR1/NF-κB)信号通路的作用。方法以(1.2、12、120)μmol/L HSYA预处理体外培养的小鼠动脉血管内皮细胞,之后以肿瘤坏死因子α(TNF-α)诱导细胞,采用Western blot法检测HSYA对TNFR1介导NF-κB信号通路相关蛋白核因子κB抑制蛋白α(IκBα)表达的影响;采用实时定量PCR检测HSYA对TNF-α诱导的NF-κB下游分子细胞间黏附分子1(ICAM-1)和E选择素(E-selectin)mRNA表达的抑制作用;采用流式细胞术检测细胞膜TNFR1表达量的变化;利用TNF-α转化酶(TACE)抑制剂TAPI-0检测TACE是否参与HSYA对TNFR1介导信号的抑制作用。结果 HSYA可呈剂量依赖性抑制TNFR1介导的NF-κB上游分子IκBα蛋白降解;HSYA可显著抑制NF-κB下游分子ICAM-1和E-selectin的mRNA表达;HSYA可显著减少细胞膜TNFR1蛋白含量;TAPI-0可抑制HSYA对TNFR1信号的调控作用。结论 HSYA可抑制血管内皮细胞中TNFR1/NF-κB信号通路而发挥抗炎作用,TACE参与介导HSYA的抗炎作用。
作者 刘金连 王海芳 马淑惠 霍雪萍 梁导艳 曹情雯 胡军 刘勤社
机构地区 陕西省人民医院中心实验室 陕西省中医研究院 西安交通大学医学部
出处 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2015年第7期945-948,共4页 Chinese Journal of Cellular and Molecular Immunology
基金 国家支撑计划(2008BAI51B01) 陕西省中医药管理局课题(13-JC028)
关键词 羟基红花黄色素A 动脉血管内皮细胞 肿瘤坏死因子α受体1 抗炎作用 肿瘤坏死因子α转化酶
分类号 R285.5 [医药卫生—中药学] R965 [医药卫生—药理学]
  • 相关文献

参考文献22

  • 1Popa C, Netea MG, van Riel PL, et al. The role of TNF-ot in chronic inllammatory conditions, intermediary metabolism, and cardiovascular risk[J]. J Lipid Res, 2007, 48(4) : 751 -762.
  • 2Zhang H, Park Y, Wu J, et al. Role of TNF-ct in vascular dysfunction [J]. Clin Sci (Lond), 2009, 116(3) : 219 -230.
  • 3Yuan W, Yang D, Sun X, et al. Effects of hydroxysafflor yellow A on proliferation and collagen synthesis of rat vascular adventitial fibroblasts induced by angiotensin II[ J ]. Int J Clin Exp Pathol, 2014, 7(9) : 5772 -5781.
  • 4Nie PH, Zhang L, Zhang WH, et al. The effects of hydroxysatllor yellow A on blood pressure and cardiac function[ J]. J Ethnophanmcl, 2012, 139 : 746 -750.
  • 5Liu YN, Zhou ZM, Chen P. Evidence that hydroxysafflor yellow A protects the heart against ischaemia-reperfusion injury by inhibiting mitochondrial permeability transition pore opening [ J ]. Clin Exp Pharmacol Physiol, 2008, 35(2) : 211 -216.
  • 6Fan L, Dang X, Shi Z, et al. Hydroxysaglor yellow A protects PC12 cells against the apoptosis induced by oxygen and glucose deprivation []]. Cell Mol Neumbiol, 2011, 31(8) : 1187 -1194.
  • 7Wu Y, Wang L, Jin M, et al. Hydroxysaffior yellow A alleviates early inflammatory response of bleomycin-induced mice lung injury [ J ]. Biol Pharm Bull, 2012, 35(4) : 515 -522.
  • 8Li J, mng S, Lu M, et al. Hydrmqnilor yellow A suppresses inflarmlatory responses c BV2 nicroglia after oxygen-glucose deprivation [ J ]. Neurosci Lett, 2013, 535:51 -56.
  • 9Shimizu N, Yoshiymm M, Omum T, et al. The ability ff hydlsamor yellow A to attenuate lipopolysaccharide-induced pulmonary inflammatory injury in mice [ J]. Phytother Res, 2010, 24 ( 12 ) : 1788 - 1795.
  • 10Jiang S, Shi Z, Li C, et al. Hydmxysaffior yellow A attenuates ischemia/ repeffusion-induced liver injury by suppressing macmphage activation [J]. Int J Clin Exp Pathol, 2014, 7(5) : 2595 -2608.

二级参考文献31

  • 1Harrison RW, Aggarwal A, Ou FS, et al. Incidence and out- comes of no-reflow phenomenon during percutaneous coronary intervention among patients with acute myocardial infarction[J]. Am J Cardiol,2013, 60(22) : 178-184.
  • 2O'Brien EC, Rose KM, Suchindran CM, et al. Temporal trends in medical therapies for ST- and non-ST elevation myo- cardial infarction: (from the Atherosclerosis Risk in Communi- ties [-ARIC] Surveillance Study [J]. Am J Cardiol, 2013, 111 (3) :305-311.
  • 3Reichlin T, Twerenbold R, Reiter M, et al. Introduction of high-sensitivity troponin assays: impact on myocardial infarc- tion incidence and prognosis[J]. Am J Med, 2012, 125 (12) : 1205-1213.
  • 4Leonardi S, Thomas L, Neely ML, et al. Comparison of the prognosis of spontaneous and pereutaneous coronary interven- tion-related myocardial infarction[J] J Am Coll Cardiol,2012, 60(22) : 2296-2304.
  • 5Levin L , Wallentin L, Bernfort L, et al. Health-related qual- ity of life of ticagrelor versus elopidogrel in patients with acute coronary syndromes-results from the PLATO trial[J]. Value Health,20a 3, 3_6(4) : 574-580.
  • 6Maisel A, Mueller C, Neath SX, et al. Copeptin helps in the early detection of patients with acute myocardial infarction: primary results of the CHOPIN trial (Copeptin helps in the early detection of patients with acute myocardial infarction) [J]. J Am Coil Cardiol,2013, 62(2) : 150-160.
  • 7Scirica BM, Kadakia MB, de Lemos JA, etal. Association be- tween natriuretic peptides and mortality among patients admit ted with myocardial infarction., a report from the ACTION Registry(R)-GWTG? [J]. Clin Chem, 2013, 59 (8) : 1205- 1214.
  • 8Patti G, Pasceri V, Mangiacapra F, et al. Efficacy of clopi- dogrel reloading in patients with acute coronary syndrome un- dergoing percutaneous coronary intervention during chronic clopidogrel therapy (from the Antiplatelet therapy for Reduc- tion of MYocardial Damage during Angioplasty [ARMYDA-8 RELOAD-ACS] triaD[J]. Am J Cardiol, 2013, 112 (2) : 162- 168.
  • 9Iida O, Soga Y, Yamauchi Y, et al. Clinical efficacy of endo- vascular therapy for patients with critical limb ischemia attrib- utable to pure isolated infrapopliteal lesions[J]. J Vasc Surg, 2013, 57(4) :974-981.
  • 10Marioni RE, Stewart MC, Murray GD, et al. Peripheral lev- els of fibrinogen, C-reactive protein, and plasma viscosity pre- dict future cognitive decline in individuals without dementia [J]. Psychosom Med, 2009, 71 (8) :901-906.

共引文献19

同被引文献346

引证文献20

二级引证文献117

细胞与分子免疫学杂志
2015年 第7期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部