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油菜素内酯对霍山石斛抗冷性能的调控效应 被引量:5

Effects of Brassinosteroids on the Chilling Resistance of Dendrobium huoshanense
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摘要 以2年生霍山石斛(Dendrobium huoshanense)的当年生茎叶为试验材料,在叶面喷施0、0.01、0.05、0.10和0.50mg·L-1油菜素内酯(BR)后,进行48h昼/夜温度为(4±1)℃/(2±1)℃的低温胁迫处理,测定其幼苗叶片叶绿素含量、抗氧化酶活性、丙二醛含量和电导率及脯氨酸含量和可溶性糖含量的变化,研究BR对霍山石斛抗冷性的影响。结果显示:(1)低温胁迫条件下,霍山石斛叶片叶绿素含量随胁迫时间推移而不断降低;SOD、POD与CAT活性变化趋势均呈单峰曲线,峰值出现在低温胁迫后10d;MDA含量和电导率均持续提高。(2)叶面喷施BR预处理显著缓解了低温胁迫对霍山石斛的伤害,显著提高其叶片叶绿素含量,显著增强了SOD、POD与CAT的活性,显著降低其MDA含量和电导率;并显著提高了叶片中脯氨酸含量与茎中可溶性糖含量。(3)BR浓度为0.10mg·L-1的处理效果显著优于其它浓度处理。研究表明,在低温胁迫条件下,油菜素内酯能够通过增强霍山石斛叶片内抗氧化酶活性,提高其渗透调节能力,有效减轻低温造成的过氧化伤害,提高叶片叶绿素含量,增强其植株的抗冷性,并以0.10mg·L-1油菜素内酯效果最佳。 In order to study the effects of different concentrations of BR on the chilling resistance of Dendrobium huoshanense,we carried out this experiment with annual stems and leaves of biennial D.huoshanense.D.huoshanense plants were sprayed by 0,0.01,0.05,0.10,0.50mg·L^-1 BR,respectively,then suffered chilling stress [day/night temperature were(4±1)℃/(2±1)℃,respectively]for 48 h.The changes of chlorophyll content,autioxidant enzyme activities,MDA content,relative conductivity,proline content in leaves and soluble sugar content in the stems of D.huoshanense seedlings were determined.The results showed that:(1)Under chilling stress,chlorophyll content was decreased constantly.The activity change trends of SOD,POD and CAT all showed unimodal curve and the peaks were all at the 10 th day after chilling stress,MDA content and relative conductivity were both increased constantly in leaves.(2)Pretreatment of leaf spraying BR significantly alleviated the hurts of chilling stress on D.huoshanense,significantly improved chlorophyll content,activities of SOD,POD and CAT,significantly reduced MDA content,relative conductivity in leaves and significantly improved proline content in leaves and soluble sugar content in the stems.(3)The effects of the treatment with BR concentration 0.10mg·L^-1 were significantly better than those of other treatments.So,BR could effectively relieve the peroxide damage to D.huoshanense plantcaused by chilling stress,improve its chlorophyll content in leaves,enhance its chilling resistance by improving autioxidant enzyme activities in leaves and their osmotic adjustment ability under chilling stress and BR concentration of 0.10mg·L^-1 was recommended.
作者 吴进东
机构地区 皖西学院
出处 《西北植物学报》 CAS CSCD 北大核心 2015年第5期985-990,共6页 Acta Botanica Boreali-Occidentalia Sinica
基金 皖西学院校级项目(WXZR1523)
关键词 油菜素内酯(BR) 低温 霍山石斛 抗冷性 brassinosteroids (BR) chilling stress Dendrobium huoshanense chilling resistance
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