摘要
目的：探讨垂体肿瘤转化基因（PTTG）mRNA和囊性纤维跨膜转运调节因子（CFTR）mRNA在慢性胰腺炎和胰腺癌疾病中的变化及临床意义。方法选择慢性胰腺炎患者20例，胰腺癌患者30例；采用实时逆转录-聚合酶链反应（RT-PCR）技术检测两组组织中PTTG mRNA和CFTR mRNA表达状况，比较分析两标志物的变化。结果慢性胰腺炎组织中CFTR mRNA的表达水平明显高于胰腺癌（P〈0.01）， PTTG mRNA在慢性胰腺炎组织中的表达水平明显低于胰腺癌（P〈0.01）。PTTG mRNA和CFTR mRNA在慢性胰腺炎和胰腺癌组织中的表达量具有相关性（r=-0.526、-0.539，P〈0.01）。结论检测PTTG mRNA表达水平可用于胰腺癌诊断，检测CFTR mRNA表达水平可用于慢性胰腺炎诊断，且均具有较强的灵敏性。
Objective To explore the application of PTTG mRNA and CFTR mRNA in the differential diagnosis of chronic pancreatitis and pancreatic cancer. Method 20 cases of patients with chronic pancreatitis and 30 cases of patients with pancreatic cancer were selected. The expression of organize PTTG mRNA and CFTRmRNA in the two groups was detected,real-time reverse transcription-polymerase chain reaction（RT-PCR） technology was applied. The changes of the two markers were comparetively analysed. Results The expression rate of CFTRmRNA in chronic pancreatitis group and PTTGmRNA in pancreatic cancer group was respectively obviously higher（P〈0.01）. The expression quantity of PTTGmRNA and CFTRmRNA in chronic pancreatitis and pancreatic cancer were in the correlation（r=-0.526,-0.539,P〈0.01）. Conclution PTTGmRNA has higher sensitivity in diagnosis of pancreatic cancer,so does CFTRmRNA in diagnosis of chronic pancreatitis. Testing organization PTTGmRNA and CFTRmRNA can be used in the differential diagnosis of chronic pancreatitis and pancreatic cancer disease.
出处
《浙江临床医学》
2015年第6期881-882,共2页
Zhejiang Clinical Medical Journal
