摘要
转录组学是功能基因组学研究中最活跃的领域之一,是一门在整体水平研究细胞中所有基因转录及转录调控规律的科学,在基础医学、生物学、微生物学和药学等领域已有广泛的应用。在中药研究领域中,该技术能从基因水平通过比较药物作用前后基因表达谱的变化对中药药理和毒理等方面进行评价。对该技术在中药研究领域的进展进行综述,主要介绍了转录组学技术在中药肝损伤筛选生物标志物和中药所致肝损伤机制研究中的应用,为该技术在中药领域的研究和发展提供有价值的参考。
As a science of cell genetic transcription and transcriptional regulation at the unitary level, transcriptomics has been applied in basic medicine, biology, microbiology, pharmacy, and so on. In the study on Chinese materia medica （CMM）, transcriptomics is used to evaluate the efficacy and toxicity by comparing the changes of gene expression profile before and after drug treatment. To provide the valuable references for the research the development of transcriptomic technology, this article reviewed the progress of its application in CMM research, especially in biomarker screening and mechanism elucidating on the hepatotoxicity induced by CMM.
出处
《中草药》
CAS
CSCD
北大核心
2015年第10期1536-1541,共6页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金资助项目(81001254)
重大新药创制科技重大专项(2013ZX09302303
2012ZX09301-001-008)
关键词
转录组学
中药肝毒性
生物标志物
RT-PCR
基因芯片
transcriptomics
hepatotoxicity induced by Chinese materia medica
biomarkers
RT-PCR
gene chip
