摘要
目的探讨反义导向分子a(Repulsive guidance molecule a,RGMa)在创伤后应激障碍模型单一延长应激模型大鼠皮质中的表达及意义。方法随机将30只Wistar大鼠分为对照组(con)、单一延长应激模型组(model)。建模后第7d观察两组大鼠行为学改变,并采用免疫荧光、逆转录PCR检测两组大鼠皮质RGMa的表达改变。结果与正常对照组比较,模型组大鼠僵立行为明显增加(<0.01),皮质RGMa蛋白及m RNA表达水平均显著增多(<0.01)。结论单一延长刺激诱导大鼠皮质RGMa蛋白及转录水平上调,可能是引起PTSD皮质体积缩小的重要分子机制之一。
Objective To explore the role of repulsive guidance molecule a（RGMa） in cortex of Wistar rats model of posttraumatic stress disorder（PTSD）. Methods 30 rats were randomly assigned into normal control and model group which was exposed to single-prolonged stress（SPS） model. The freezing behavior of rats was observed and the expression of RGMa in cortex were examined by immunofluorescence and reverse transcription-polymerase chain reaction（RT-PCR）. Results The freezing behavior was increased in model group than in control group（P〈0.01）. The expression levels of RGMa protein and mRNA in cortex were increase markedly in model group than in the normal control group（P〈0.01）. Conclusion The upregulated expression of RGMa protein and mRNA in cortex by SPS might be related to the reduced cortex volume of PTSD.
出处
《解剖科学进展》
CAS
2015年第3期260-262,共3页
Progress of Anatomical Sciences
基金
国家自然科学基金资助项目(No.81172409)
